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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 22号
  1. 部局別インデックス
  2. 農学部

2,4 -ジニトロフェニルヒドラジンを用いるタウリンーピルビン酸トランスアミナーゼの活性測定法(農芸化学科)

http://hdl.handle.net/20.500.12000/4333
http://hdl.handle.net/20.500.12000/4333
3956750e-5ee9-4a25-b2a7-f3d350b82eee
名前 / ファイル ライセンス アクション
KJ00000162518.pdf KJ00000162518.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル 2,4 -ジニトロフェニルヒドラジンを用いるタウリンーピルビン酸トランスアミナーゼの活性測定法(農芸化学科)
言語 ja
作成者 当山, 清善

× 当山, 清善

ja 当山, 清善

安田, 正昭

× 安田, 正昭

ja 安田, 正昭

左右田, 健次

× 左右田, 健次

ja 左右田, 健次

Toyama, Seizen

× Toyama, Seizen

en Toyama, Seizen

Yasuda, Masaaki

× Yasuda, Masaaki

en Yasuda, Masaaki

Soda, Kenji

× Soda, Kenji

en Soda, Kenji

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 タウリンーピルビン酸トランスアミナーゼ活性を2,4-ジニトロフェニルヒドラゾンを用いて測定する方法を検討し, 次の結果を得た。酵素反応混液のピルビン酸とスルホアセトアルデヒドを2,4-ジニトロフェニルヒドラゾンとした後トルオールで抽出すると, ピルビン酸のヒドラゾンはトルオール層に抽出され, スルホアセトアルデヒドのヒドラゾンは水層に残留する。水層中のスルホアセトアルデヒドヒドラゾンはアルカリ性で発色させると435mμに吸収極大を有する。酵素活性は本吸収極大の吸光度を定量することによって測定し得る。
内容記述タイプ Other
内容記述 This communication describes an assay method of taurine : pyruvate transaminase with 2,4- dinitrophenylhydrazine. The results obtained were as follows; Pyruvic acid and sulfoacetaldehyde in the enzyme reaction mixture were allowed to react with 2,4- dinitrophenylhydrazine, after which the hydrazones formed were separated from each other with toluol. The pyruvic acid hydrazone was extracted to the toluol layer and the sulfoacetaldehyde hydrazone remained in the aqueous layer. The sulfoacetaldehyde hydrazone of the aqueous layer gave an intense red brown color with the mixture of NaOH and Na_2CO_3 solution, exhibiting the absorption maximum at 435mμ. The transaminase activity could be determined by measuring the absorbance at the absorption peak.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4333
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 22, p. 215-223
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