{"_buckets": {"deposit": "398c5129-1b5b-4f52-b299-fc0d9d0fc019"}, "_deposit": {"id": "2003325", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003325"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003325", "sets": ["1642837909579", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "2,4 -\u30b8\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30d2\u30c9\u30e9\u30b8\u30f3\u3092\u7528\u3044\u308b\u30bf\u30a6\u30ea\u30f3\u30fc\u30d4\u30eb\u30d3\u30f3\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u6d3b\u6027\u6e2c\u5b9a\u6cd5(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "An assay method of taurine-pyruvate transaminase activity with 2,4- dinitrophenylhydrazine (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5f53\u5c71, \u6e05\u5584", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5b89\u7530, \u6b63\u662d", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5de6\u53f3\u7530, \u5065\u6b21", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Toyama, Seizen", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yasuda, Masaaki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Soda, Kenji", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u30bf\u30a6\u30ea\u30f3\u30fc\u30d4\u30eb\u30d3\u30f3\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u6d3b\u6027\u30922,4-\u30b8\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30d2\u30c9\u30e9\u30be\u30f3\u3092\u7528\u3044\u3066\u6e2c\u5b9a\u3059\u308b\u65b9\u6cd5\u3092\u691c\u8a0e\u3057, \u6b21\u306e\u7d50\u679c\u3092\u5f97\u305f\u3002\u9175\u7d20\u53cd\u5fdc\u6df7\u6db2\u306e\u30d4\u30eb\u30d3\u30f3\u9178\u3068\u30b9\u30eb\u30db\u30a2\u30bb\u30c8\u30a2\u30eb\u30c7\u30d2\u30c9\u30922,4-\u30b8\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30d2\u30c9\u30e9\u30be\u30f3\u3068\u3057\u305f\u5f8c\u30c8\u30eb\u30aa\u30fc\u30eb\u3067\u62bd\u51fa\u3059\u308b\u3068, \u30d4\u30eb\u30d3\u30f3\u9178\u306e\u30d2\u30c9\u30e9\u30be\u30f3\u306f\u30c8\u30eb\u30aa\u30fc\u30eb\u5c64\u306b\u62bd\u51fa\u3055\u308c, \u30b9\u30eb\u30db\u30a2\u30bb\u30c8\u30a2\u30eb\u30c7\u30d2\u30c9\u306e\u30d2\u30c9\u30e9\u30be\u30f3\u306f\u6c34\u5c64\u306b\u6b8b\u7559\u3059\u308b\u3002\u6c34\u5c64\u4e2d\u306e\u30b9\u30eb\u30db\u30a2\u30bb\u30c8\u30a2\u30eb\u30c7\u30d2\u30c9\u30d2\u30c9\u30e9\u30be\u30f3\u306f\u30a2\u30eb\u30ab\u30ea\u6027\u3067\u767a\u8272\u3055\u305b\u308b\u3068435m\u03bc\u306b\u5438\u53ce\u6975\u5927\u3092\u6709\u3059\u308b\u3002\u9175\u7d20\u6d3b\u6027\u306f\u672c\u5438\u53ce\u6975\u5927\u306e\u5438\u5149\u5ea6\u3092\u5b9a\u91cf\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u6e2c\u5b9a\u3057\u5f97\u308b\u3002", "subitem_description_type": "Other"}, {"subitem_description": "This communication describes an assay method of taurine : pyruvate transaminase with 2,4- dinitrophenylhydrazine. The results obtained were as follows; Pyruvic acid and sulfoacetaldehyde in the enzyme reaction mixture were allowed to react with 2,4- dinitrophenylhydrazine, after which the hydrazones formed were separated from each other with toluol. The pyruvic acid hydrazone was extracted to the toluol layer and the sulfoacetaldehyde hydrazone remained in the aqueous layer. The sulfoacetaldehyde hydrazone of the aqueous layer gave an intense red brown color with the mixture of NaOH and Na_2CO_3 solution, exhibiting the absorption maximum at 435m\u03bc. The transaminase activity could be determined by measuring the absorbance at the absorption peak.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4333"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "22", "bibliographicPageEnd": "223", "bibliographicPageStart": "215"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162518.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003325/files/KJ00000162518.pdf"}, "version_id": "dce80ba8-6a6b-481b-b6b8-2e2655de0938"}]}, "item_title": "2,4 -\u30b8\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30d2\u30c9\u30e9\u30b8\u30f3\u3092\u7528\u3044\u308b\u30bf\u30a6\u30ea\u30f3\u30fc\u30d4\u30eb\u30d3\u30f3\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u6d3b\u6027\u6e2c\u5b9a\u6cd5(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837909579", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4333", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003325", "relation": {}, "relation_version_is_last": true, "title": ["2,4 -\u30b8\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30d2\u30c9\u30e9\u30b8\u30f3\u3092\u7528\u3044\u308b\u30bf\u30a6\u30ea\u30f3\u30fc\u30d4\u30eb\u30d3\u30f3\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u6d3b\u6027\u6e2c\u5b9a\u6cd5(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}