Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2009-05-28 |
タイトル |
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タイトル |
NF-kappaB activation by Helicobacter pylori requires Akt-mediated phosphorylation of p65 |
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言語 |
en |
作成者 |
Takeshima, Eriko
Tomimori, Koh
Kawakami, Hirochika
Ishikawa, Chie
Sawada, Shigeki
Tomita, Mariko
Senba, Masachika
Kinjo, Fukunori
Mimuro, Hitomi
Sasakawa, Chihiro
Fujita, Jiro
Mori, Naoki
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
[Background] The inflammatory response in Helicobacter pylori-infected gastric tissue is mediated by cag pathogenicity island (PAI)-dependent activation of nuclear factor-κB (NF-κB). Phosphatidylinositol 3-kinase (PI3K)/Akt signaling is known to play a role in NF-κB activation, but little information is available on the relationship between H. pylori and PI3K/Akt signaling in gastric epithelial cells. We examined whether H. pylori activates Akt in gastric epithelial cells, the role of cag PAI in this process and the role of Akt in regulating H. pylori-induced NF-κB activation. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
[Results] Phosphorylated Akt was detected in epithelial cells of H. pylori-positive gastric tissues. Although Akt was activated in MKN45 and AGS cells by coculture with cag PAI-positive H. pylori strains, a cag PAI-negative mutant showed no activation of Akt. H. pylori also induced p65 phosphorylation. PI3K inhibitor suppressed H. pylori-induced p65 phosphorylation and NF-κB transactivation, as well as interleukin-8 expression. Furthermore, transfection with a dominant-negative Akt inhibited H. pylori-induced NF-κB transactivation. Transfection with small interference RNAs for p65 and Akt also inhibited H. pylori-induced interleukin-8 expression. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
[Conclusion] The results suggest that cag PAI-positive H. pylori activates Akt in gastric epithelial cells and this may contribute to H. pylori-mediated NF-κB activation associated with mucosal inflammation and carcinogenesis. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
BioMed Central Ltd. |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/10318 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
URI |
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関連識別子 |
http://www.biomedcentral.com/1471-2180/9/36 |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1186/1471-2180-9-36 |
関連情報 |
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識別子タイプ |
PMID |
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関連識別子 |
19216748 |
収録物識別子 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12035256 |
収録物名 |
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言語 |
en |
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収録物名 |
BMC microbiology |
書誌情報 |
巻 9,
p. 36,
発行日 2009-02-12
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