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"subitem_description_type": "Other"}, {"subitem_description": "\u8981\u7d04\uff08\u6b27\u6587\uff09:I have previously isolated a fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA(Okinawamozuku), the yield of which was estimated to be 2.0-2.3%(wet seaweed). Fucoidan has physiological and biological activities of an cholesterol lowering, anti-coagulant and anti-tumor agent. I report herein rheological properties and blood anti-coagulant activity of the fucoidan prepared from commercially cultured Okinawamozuku on nets at sea off the southern part of Okinawa Island(Chinen Village),Japan. The fucoidan consisted of L-fucose:D-xylose:D-glucuronic acid:acetic acid:sulfuric acid=4.0:0.1:1.0:2.0:2.0. The molecular weight of the fucoidan was estimated to be 500,000. The non-Newtonian behavior and dynamic viscoelasticity of the fucoidan were measured with a rheogoniometer equipped with a coaxial cylinder and rotating outer cylinder. The flow curves of the fucoidan, at 25\u2103, showed shear-thinning behavior below 1.5%, but plastic behavior at 2.0%. A little higher dynamic viscosity was observed than that of storage modulus in fucoidan solutions at various concentrations. The dynamic viscoelasticity increased with addition of NaCl(0.1%) and CaCl_2(0.1%) to 2.0% fucoidan solution. The dynamic viscoelasticity of the fucoidan was stable over a wide pH range. The fucoidan had unique Theological characteristics in aqueous solution, therefore it migh be able to use in foods, cosmetics and some other industries. Blood anti-coagulant activity tests were conducted on th fucoidan with human plasma in comparison with heparin. The thrombin and activated thromboplastin activity was higher than that of heparin. Partially hydrolyzed fucoidans with sulfuric acid and formic acid had higher activity that that of native fucoidan.", "subitem_description_type": "Other"}, {"subitem_description": "\u672a\u516c\u958b\uff1aP.59\u4ee5\u964d\uff08\u5225\u5237\u8ad6\u6587\u306e\u305f\u3081\uff09", "subitem_description_type": "Other"}, {"subitem_description": "\u7814\u7a76\u5831\u544a\u66f8", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7530\u5e78\u6b63\u90a6"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/13651"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "NCID", "subitem_1522646572813": "BA6736950X"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-03", "bibliographicIssueDateType": "Issued"}}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "14560105.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2005094/files/14560105.pdf"}, "version_id": "1c34e067-1b66-495b-8762-4f6eced5f583"}]}, "item_title": "\u990a\u6b96\u30e2\u30ba\u30af\u304b\u3089\u88fd\u9020\u3057\u305f\u30d5\u30b3\u30a4\u30c0\u30f3\u306e\u6a5f\u80fd\u30fb\u7279\u6027\u306e\u958b\u767a\u306b\u95a2\u3059\u308b\u7814\u7a76", "item_type_id": "15", "owner": "1", "path": ["1642838403123", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/13651", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2009-12-07"}, "publish_date": "2009-12-07", "publish_status": "0", "recid": "2005094", "relation": {}, "relation_version_is_last": true, "title": ["\u990a\u6b96\u30e2\u30ba\u30af\u304b\u3089\u88fd\u9020\u3057\u305f\u30d5\u30b3\u30a4\u30c0\u30f3\u306e\u6a5f\u80fd\u30fb\u7279\u6027\u306e\u958b\u767a\u306b\u95a2\u3059\u308b\u7814\u7a76"], "weko_shared_id": -1}
  1. その他
  1. 部局別インデックス
  2. 農学部

養殖モズクから製造したフコイダンの機能・特性の開発に関する研究

http://hdl.handle.net/20.500.12000/13651
http://hdl.handle.net/20.500.12000/13651
c2cb864c-e9cb-42f3-aef1-c83a9a945eb0
名前 / ファイル ライセンス アクション
14560105.pdf 14560105.pdf
Item type デフォルトアイテムタイプ（フル）(1)
公開日 2009-12-07
タイトル
タイトル 養殖モズクから製造したフコイダンの機能・特性の開発に関する研究
言語 ja
作成者 田幸, 正邦

× 田幸, 正邦

ja 田幸, 正邦
Tako, Masakuni

× Tako, Masakuni

en Tako, Masakuni
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
主題
言語 ja
主題Scheme Other
主題 フコイダン
言語 ja
主題Scheme Other
主題 脱アセチルフコイダン
言語 ja
主題Scheme Other
主題 オキナワモズク
言語 ja
主題Scheme Other
主題 イトモズク
言語 ja
主題Scheme Other
主題 流動特性
言語 ja
主題Scheme Other
主題 動的粘弾性
言語 ja
主題Scheme Other
主題 抗血液凝固作用
言語 ja
主題Scheme Other
主題 機能・特性
言語 ja
主題Scheme Other
主題 Function・Characteristics
内容記述
内容記述タイプ Other
内容記述 科研費番号: 14560105
内容記述タイプ Other
内容記述 平成14－15年度科学研究費補助金(一般基盤研究C)研究成果報告書
内容記述タイプ Other
内容記述 研究概要:先に、沖縄県で養殖により生産されているオキナワモズクに、フコイダンが多量含まれる事を明らかにした。また、このフコイダンは酢酸基を置換する事を明らかにした。ここでは、最初に、本藻から製造したフコイダン水溶液の流動特性と動的粘弾性をレオゴニオメーターを使用して検討した。フコイダン水溶液は濃度1.5%まで擬塑性流動を、2.0%では降伏値が認められ、塑性流動を示した。本フコイダンの動的粘弾性は濃度の増大に伴って1.0%まで徐々に増大したが、それ以上の濃度で急激に増大した。動的弾性率に比較して粘性率の値が高かった。塩化ナトリウムおよび塩化カルシウムの添加によって動的弾性率が増大した。また、本フコイダンの動的粘弾性は、広いpH域で高い値を示した。以上の結果から、オキナワモズクから製造したフコイダンは、流動性に優れている事が解った。従って、食品、化粧品およびその他の工業で利用可能である事が示唆された。次に、オキナワモズクに加え、沖縄県で養殖により生産されているイトモズクからフコイダンを製造してそれらの抗血液(ヒト)凝固作用について調べた。なお、オキナワモズクから製造したフコイダンは脱アセチル化物についても調べた。すでに抗血液凝固剤として使用されているヘパリンを対照に比較検討した。オキナワモズクおよびイトモズクから製造したフコイダンのトロンボプラスチン活性は、ヘパリンのそれと比較して高い値を示した。またトロンビン活性も高かった。フコイダンを脱アセチル化すると、抗血液凝固作用が増大した。以上の結果から、オキナワモズクおよびイトモズクから製造したフコイダンは血液の凝固を阻止する作用のある事が解った。フコイダンは多くの機能を有する事から現在最も注目されている多糖である。特に、オキナワモズクに含まれるフコイダンは極めて特異な機能を有する可能性がある。
内容記述タイプ Other
内容記述 要約（欧文）:I have previously isolated a fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA(Okinawamozuku), the yield of which was estimated to be 2.0-2.3%(wet seaweed). Fucoidan has physiological and biological activities of an cholesterol lowering, anti-coagulant and anti-tumor agent. I report herein rheological properties and blood anti-coagulant activity of the fucoidan prepared from commercially cultured Okinawamozuku on nets at sea off the southern part of Okinawa Island(Chinen Village),Japan. The fucoidan consisted of L-fucose:D-xylose:D-glucuronic acid:acetic acid:sulfuric acid=4.0:0.1:1.0:2.0:2.0. The molecular weight of the fucoidan was estimated to be 500,000. The non-Newtonian behavior and dynamic viscoelasticity of the fucoidan were measured with a rheogoniometer equipped with a coaxial cylinder and rotating outer cylinder. The flow curves of the fucoidan, at 25℃, showed shear-thinning behavior below 1.5%, but plastic behavior at 2.0%. A little higher dynamic viscosity was observed than that of storage modulus in fucoidan solutions at various concentrations. The dynamic viscoelasticity increased with addition of NaCl(0.1%) and CaCl_2(0.1%) to 2.0% fucoidan solution. The dynamic viscoelasticity of the fucoidan was stable over a wide pH range. The fucoidan had unique Theological characteristics in aqueous solution, therefore it migh be able to use in foods, cosmetics and some other industries. Blood anti-coagulant activity tests were conducted on th fucoidan with human plasma in comparison with heparin. The thrombin and activated thromboplastin activity was higher than that of heparin. Partially hydrolyzed fucoidans with sulfuric acid and formic acid had higher activity that that of native fucoidan.
内容記述タイプ Other
内容記述 未公開：P.59以降（別刷論文のため）
内容記述タイプ Other
内容記述 研究報告書
出版者
言語 ja
出版者 田幸正邦
言語
言語 jpn
資源タイプ
資源タイプ research report
資源タイプ識別子 http://purl.org/coar/resource_type/c_18ws
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/13651
識別子タイプ HDL
収録物識別子
収録物識別子タイプ NCID
収録物識別子 BA6736950X
書誌情報
発行日 2004-03
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