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2)\u67d3\u8272\u4f53\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u306e\u5fdc\u7528\u6539\u5909(\u633f\u5165)\u3092\u884c\u3063\u305f\u30df\u30cb\u67d3\u8272\u4f53\u306e\u3001\u5bbf\u4e3b\u7d30\u80de\u3067\u3042\u308bDT40\u304b\u3089\u4ed6\u306e\u7d30\u80de\u3078\u306e\u79fb\u5165\u3092\u884c\u3063\u305f\u3002\u30d2\u30c8HPRT\u907a\u4f1d\u5b50\u3092\u3082\u3064\u30df\u30cb\u67d3\u8272\u4f53\u3092\u5fae\u5c0f\u6838\u878d\u5408\u6cd5\u306b\u3088\u308a\u3001\u7dda\u7dad\u8089\u816b\u7d30\u80de\u4e9c\u682a(HT1080,HPRT(-))\u3078\u79fb\u5165\u3057\u305f\u3002G418\u8010\u6027\u3092\u6307\u6a19\u306b\u30af\u30ed\u30fc\u30f3 (HT1080,HPRT(-))\u3092\u691c\u8a0e\u3057\u305f\u3068\u3053\u308d\u3001\u30df\u30cb\u67d3\u8272\u4f53\u306f\u3001\u7d30\u80de\u3042\u305f\u308a\u306e\u30b3\u30d4\u30fc\u6570\u306b\u5909\u52d5\u304c\u898b\u3089\u308c\u308b(1\uff5e8\u30b3\u30d4\u30fc)\u3082\u306e\u306e\u3001\u5b89\u5b9a\u306b\u5b58\u5728\u3057\u3066\u3044\u305f\u3002\u307e\u305f\u3001HPRT\u907a\u4f1d\u5b50\u767a\u73fe\u3082\u52b9\u7387\u3088\u304f\u8a8d\u3081\u3089\u308c\u305f\u3002\u3088\u3063\u3066\u3001\u3053\u306e\u30df\u30cb\u67d3\u8272\u4f53\u306f\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u305f\u3081\u306e\u67d3\u8272\u4f53\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u6709\u52b9\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "\u8981\u7d04(\u6b27\u6587):We have established an efficient system for introducing a whole BAC clone including an intact gene unit onto a minichromosome based on the human X chromosome. The use of mutant loxP sites with Cre recombinase and direct selection for the retrofitting event led to a high efficiency of correctly retrofitted clones. A modified BAC containing the intact human HPRT gene was correctly retrofitted onto the minichromosome in DT40 cells in approximately 75% of analysed clones. Modification of BACs for retrofitting was used method previously reported (Kaname and Huxley, BioTechniques 31:273(2001)). For another BAC clone containing human factor IX gene, the efficiency of retrofitting was about 80%. For a plasmid (4.5kb) having a neoR expression unit, the retrofitting efficiency was 100%. The HPRT gene is expressed from the minichromosome at approximately the same level in both of these clones. This system allows one to introduce any gene or gene cluster which can be cloned as a BAC into the minichromosome for gene delivery. We also tried to transfer the modified minichromosomes into other cells (HT1080,HPRT(-)) by microcell-fusion method. It was efficient to isolate the transchromosomic cells by screening of G418 resistant clones. Of the resistant clones, copy number of the minichromosome per cell was in range of 1-8. Its expression was, however, efficient and stable. Thus, the minichromosome would be used as a chromosome vector for gene therapy.", "subitem_description_type": "Other"}, {"subitem_description": "\u672a\u516c\u958b\uff1aP.9\u4ee5\u964d(\u5225\u5237\u8ad6\u6587\u306e\u305f\u3081)", "subitem_description_type": "Other"}, {"subitem_description": "\u7814\u7a76\u5831\u544a\u66f8", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u8981\u5321"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/13990"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "NCID", "subitem_1522646572813": "BA74136818"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-03", "bibliographicIssueDateType": "Issued"}}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "15590293.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2005151/files/15590293.pdf"}, "version_id": "0113b82d-7b8a-48d3-af2e-338772ee2e25"}]}, "item_title": "\u30df\u30cb\u67d3\u8272\u4f53\u3092\u7528\u3044\u305f\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u305f\u3081\u306e\u3001\u30d2\u30c8\u4eba\u53e3\u67d3\u8272\u4f53\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9\u3068\u5fdc\u7528", "item_type_id": "15", "owner": "1", "path": ["1642838403123", "1642838407795"], "permalink_uri": "http://hdl.handle.net/20.500.12000/13990", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2009-12-16"}, "publish_date": "2009-12-16", "publish_status": "0", "recid": "2005151", "relation": {}, "relation_version_is_last": true, "title": ["\u30df\u30cb\u67d3\u8272\u4f53\u3092\u7528\u3044\u305f\u3001\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u305f\u3081\u306e\u3001\u30d2\u30c8\u4eba\u53e3\u67d3\u8272\u4f53\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9\u3068\u5fdc\u7528"], "weko_shared_id": -1}