Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2010-03-11 |
タイトル |
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タイトル |
Molecular characterization of Legionella pneumophila-induced interleukin-8 expression in T cells |
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言語 |
en |
作成者 |
Takamatsu, Reika
Teruya, Hiromitsu
Takeshima, Eriko
Ishikawa, Chie
Matsumoto, Kunihiro
Mukaida, Naofumi
Li, Jian-Dong
Heuner, Klaus
Higa, Futoshi
Fujita, Jiro
Mori, Naoki
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
[Background]Legionella pneumophila is the causative agent of human Legionnaire's disease. During infection, the bacterium invades macrophages and lung epithelial cells, and replicates intracellularly. However, little is known about its interaction with T cells. We investigated the ability of L. pneumophila to infect and stimulate the production of interleukin-8 (IL-8) in T cells. The objective of this study was to assess whether L. pneumophila interferes with the immune system by interacting and infecting T cells. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
[Results]Wild-type L. pneumophila and flagellin-deficient Legionella, but not L. pneumophila lacking a functional type IV secretion system Dot/Icm, replicated in T cells. On the other hand, wild-type L. pneumophila and Dot/Icm-deficient Legionella, but not flagellin-deficient Legionella or heat-killed Legionella induced IL-8 expression. L. pneumophila activated an IL-8 promoter through the NF-κB and AP-1 binding regions. Wild-type L. pneumophila but not flagellin-deficient Legionella activated NF-κB, p38 mitogen-activated protein kinase (MAPK), Jun N-terminal kinase (JNK), and transforming growth factor β-associated kinase 1 (TAK1). Transfection of dominant negative mutants of IκBα, IκB kinase, NF-κB-inducing kinase, TAK1, MyD88, and p38 MAPK inhibited L. pneumophila-induced IL-8 activation. Inhibitors of NF-κB, p38 MAPK, and JNK blocked L. pneumophila-induced IL-8 expression. In addition, c-Jun, JunD, cyclic AMP response element binding protein, and activating transcription factor 1, which are substrates of p38 MAPK and JNK, bound to the AP-1 site of the IL-8 promoter. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
[Conclusions]Taken together, L. pneumophila induced a flagellin-dependent activation of TAK1, p38 MAPK, and JNK, as well as NF-κB and AP-1, which resulted in IL-8 production in human T cells, presumably contributing to the immune response in Legionnaire's disease. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
BioMed Central Ltd. |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/16267 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
URI |
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関連識別子 |
http://www.biomedcentral.com/1471-2180/10/1 |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1186/1471-2180-10-1 |
関連情報 |
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識別子タイプ |
PMID |
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関連識別子 |
18034886 |
収録物識別子 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12035256 |
収録物名 |
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言語 |
en |
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収録物名 |
BMC microbiology |
書誌情報 |
巻 10,
p. 1,
発行日 2010-01-05
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