{"_buckets": {"deposit": "5074d2f1-f28c-44d0-befa-0979b9e8d529"}, "_deposit": {"id": "2005662", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2005662"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02005662", "sets": ["1642838403123", "1642838407795"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "ヘモグロビンによるキジ亜科の系統的研究", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Phylogenetic relationship of Galliform subfamilies by hemoglobin", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "江口, 幸典", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "嘉陽, 進", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Eguchi, Yukinori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kayo, Susumu", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "ヘモグロビン"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "アミノ酸配列"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "分子進化"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "系統樹"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "キジ亜科"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Hemoglobin"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Amino Acid Sequence"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Molecular Evolution"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Phylogenetic Tree"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "キジ科"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "科研費番号: 07804061", "subitem_description_type": "Other"}, {"subitem_description": "平成7年度～平成8年度科学研究費補助金(基盤研究(C))研究成果報告書", "subitem_description_type": "Other"}, {"subitem_description": "研究概要（和文）：キジ亜科間の系統関係については、形態学的研究を初め、卵白リゾチーム、DNAハイブリダイゼーション法及びミトコンドリアの塩基配列を基に解析が行われているが、その結果は異なった系統樹を示してきた。そこで生命維持に不可欠なヘモグロビンの配列を決定し、先の結果と比較検討する事を試みた。最初の計画では、血液の入手が困難であることも考慮しPCRを多用する予定であったが、幸いにも血液が入手できたので、今までのデータの蓄積等を考慮し、まずアミノ酸配列の決定を行い、キジ亜科間の系統関係を明らかにした。その結果、ミトコンドリアの塩基配列とは似ているが、アメリカウズラ亜科とホロホロ鳥亜科の関係が逆転している系統樹を得ることができた。この分子系統樹の結果は、現在得られている化石のデータとも一致しているが、残念な事に、アメリカウズラ亜科の分岐に関する化石は発見されていない。今回得られた系統樹をより確信できるよう、若干のアメリカウズラ亜科の種を加え再検討すべきであると考えている。また、これらの解析等と平行して、稀少種への対応の為に羽よりPCR反応ができないかについて検討を重ねてきた。これまでの抽出方法では、DNAの抽出効率が悪いためミトコンドリアDNAの様な多コピー性の領域は、比較的簡単に増幅することが可能であった。しかしミトコンドリア以外の領域を増幅する為には多少問題があるため。DNA抽出方法を改良し、効率よく抽出できるようになった。そこでこれまでに得られたキジ亜科の羽よりDNAを抽出し、PCR反応により増幅する事が可能になり、塩基配列の決定までを行えるようになった。", "subitem_description_type": "Other"}, {"subitem_description": "概要(英文)：Phylogenetic relationships of Galliform subfamilies analyzed by morphological classification, DNA hybridization, Lysozyme c amino acid sequence data and cytochrome b nucleic acid sequence data. However, these results were showed the different phylogenetic trees. We approach phylogenetic relationship of subfamilies of Galliforms by hemoglobin amino acid sequence data. In the first plan, it considered that the acquirement of the avian blood was difficult, it planned to use PCR. The blood of California quail and Guinea fowls could be luckily, acquired. It considered that we got the amino acid sequence data of some spices of Galliforms. We determined the hemoglobin amino acid sequence of California quail and Guinea fowls. The amino acid sequence data of some spices of Galliforms, added two new data of California quail and Guinea fowls, analyzed by maximum likelihood method and neighbor joining method. As a result, phylogenetic tree from hemoglobin data resembled tree from the mitochondria DNA data and DNA hybridization. These two trees were reversed the relationship between California quail and Guinea fowls. In parallel of these study, we developed the PCR application for the rare species which difficult to get the blood. We improved a new method of DNA extraction from the feather which got high yield as the conventional method. Recently, we extracted DNA from the chicken feather and the PCR reaction can amplify of globin gene.", "subitem_description_type": "Other"}, {"subitem_description": "研究報告書", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "江口幸典"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/18189"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "NCID", "subitem_1522646572813": "BA35379536"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1997-03", "bibliographicIssueDateType": "Issued"}}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "AM", "subitem_1600292170262": "http://purl.org/coar/version/c_ab4af688f83e57aa"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "07804061.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2005662/files/07804061.pdf"}, "version_id": "cf5d313c-4564-4d82-8227-53263b304c2c"}]}, "item_title": "ヘモグロビンによるキジ亜科の系統的研究", "item_type_id": "15", "owner": "1", "path": ["1642838403123", "1642838407795"], "permalink_uri": "http://hdl.handle.net/20.500.12000/18189", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-10-05"}, "publish_date": "2010-10-05", "publish_status": "0", "recid": "2005662", "relation": {}, "relation_version_is_last": true, "title": ["ヘモグロビンによるキジ亜科の系統的研究"], "weko_shared_id": -1}