{"_buckets": {"deposit": "a5d2962d-5b82-4559-a2ff-6f4d996f0392"}, "_deposit": {"id": "2005727", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2005727"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02005727", "sets": ["1642838403123", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "琉球列島に分布するマングローブ樹種の集団遺伝学的解析に関する基礎的研究", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Fundamental Study of Population Genetics of Magngrove Tree Species in Ryukyu Islands.", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "馬場, 繁幸", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "中須賀, 常雄", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "佐藤, 一紘", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Baba, Shigeyuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nakasuga, Tsuneo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sato, Kazuhiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Electrophoresis"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Intraspecific Variation"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Isozyme"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Kin-ship Analysis"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Mangroves"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Population Genetics"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Ryukyu Islands"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Zymogram"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "アイソザイム"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "ザイモグラム"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "マングローブ"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "家系分析"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "琉球列島"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "種内変異"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "集団遺伝学"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "電気泳動"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "科研費番号: 63480063", "subitem_description_type": "Other"}, {"subitem_description": "昭和63年度～平成2年度科学研究費補助金(一般研究(B))研究成果報告書", "subitem_description_type": "Other"}, {"subitem_description": "研究概要(和文)：研究代表者と研究分担者は、琉球列島に分布するマングローブ林の主要構成樹種であるオヒルギ(Bruguiera gymnorrhiza)、メヒルギ(Kandelia candel)、ヤエヤマヒルギ(Rhizophora stylosa)を主要な研究材料とし、研究実施計画に基づき研究を行った。その研究結果の概要は、次の通りである。1.ポリアクリルアミド垂直スラブ電気泳動法を用いたアイソザイムの染色方法、すなわちザイモグラム化法の改良に努め、染色を試みた16酵素種の中で、オヒルギ、メヒルギでは11酵素種、ヤエヤマヒルギでは10酵素種のザイモグラム化が可能となり、前年度まで以上に集団内の遣伝的変異の解析精度が向上した。2.解析精度が向上したので、ホスホグルコムターゼの遺伝子座と対立遺伝子が推定され、オヒルギではホスホグルコムターゼには2遺伝子座があり、石垣島の宮良川の集団、西表島の船浦湾と浦内川の2集団、沖縄島の億首川の集団の計4集団間の遺伝子の構成が異なっていること、特に沖縄島の集団は、石垣島および西表島の集団に比較し、遺伝子構成が大きく異なっていることが明らかにされた。3.メヒルギでは西表島のヤシ川の河口にはじまり、上流域に600m以上に渡って分布していた全ての個体 (229個体)の分布位置がマッピングされ、全個体のパーオキシダーゼの表現型を用いた家系分析が行なわれた。4.メヒルギの家系分析から、河川の流れや潮の干満による胎生種子の移動がこれまで以上に明らかにされ、しかも上流域、中流域、河口域での家系群の構成割合が異なっていたことから、胎生種子の移動がランダムに生じていないこと、小さな河川に沿った集団であっても、交配がランダムに行われていないことが明らかにされた。5.外部形態(主に葉の外部形態)の変異が明らかにされたが、マングローブ樹種でも、外部形態の変異の解析より以上に、アイソザイムを用いた遺伝的変異の解析が、より有効であることが明らかにされた。", "subitem_description_type": "Other"}, {"subitem_description": "研究概要(英文)：The word \"mangrove\" usually points out constituent plants growing in the intertidal zones and the estuaries in tropics and sub-tropics. Therefore, \"mangrove\" includes more than one hundred plant species. Mangroves, that means mangrove plant species, are widely distributed in intertidal zones and estuaries in tropics and sub-tropics. Only seven major components of mangroves are distributed in Ryukyu islands, because the location of the islands is near the northern limit of their distributions. The most of studies concerning with mangroves have been done in the fields of ecology, plant taxonomy, silviculture and so on, but few in the genetical studies. An attempt of this study was made to get and to examine the possibility of application of population genetics of some mangrove tree species distributing in Ryukyu islands. For the analysis of genetic diversities of mangroves, the methods of electrophoresis with discontinuous, vertical slab gel were applied, then those were modified successfully to apply to mangroves. 16 enzyme species were tries to stain, but 11 zymogrames of 16 enzyme species were gotten for Bruguiera gymnorrhiza and Kandelia candel, and 10 of those were gotten for Rhizophora stylosa. Two phosphoglucomutase loci of B. gymnorrhiza were detected, and five alleles of those two loci were suspected. Genetic components of B. gymnorrhiza were compared among four populations, one in Okinawa islands, one in Ishigaki island and two in Iriomote island. The components of those were different, but the differentiations correlated with topographic distances among them. Kin-ship analysis of K. candel were carried out for using peroxidase phenotypes of 229 individuals distributing along Yashi river in Iriomote island, then dispersion dynamics of those viviparous seeds were clearly explained. More detailed studies should be carried out with analysis of many more enzyme species, individuals and sites of mangroves in Ryukyu islands, because this study is the first one of the genetic diversities and population genetics in Japan.", "subitem_description_type": "Other"}, {"subitem_description": "未公開：P.4～P.72(別刷論文のため)", "subitem_description_type": "Other"}, {"subitem_description": "研究報告書", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "馬場繁幸"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/18687"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "NCID", "subitem_1522646572813": "BA74757269"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1991-03", "bibliographicIssueDateType": "Issued"}}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "63480063.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2005727/files/63480063.pdf"}, "version_id": "98b75976-4f4e-4732-aa0c-fb2ca3c1bf22"}]}, "item_title": "琉球列島に分布するマングローブ樹種の集団遺伝学的解析に関する基礎的研究", "item_type_id": "15", "owner": "1", "path": ["1642838403123", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/18687", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2011-02-01"}, "publish_date": "2011-02-01", "publish_status": "0", "recid": "2005727", "relation": {}, "relation_version_is_last": true, "title": ["琉球列島に分布するマングローブ樹種の集団遺伝学的解析に関する基礎的研究"], "weko_shared_id": -1}