| Item type |
デフォルトアイテムタイプ（フル）(1) |
| 公開日 |
2018-01-30 |
| タイトル |
|
|
タイトル |
Atribacteria from the Subseafloor Sedimentary Biosphere Disperse to the Hydrosphere through Submarine Mud Volcanoes |
|
言語 |
en |
| 作成者 |
Hoshino, Tatsuhiko
Toki, Tomohiro
Ijiri, Akira
Morono, Yuki
Machiyama, Hideaki
Ashi, Juichiro
Okamura, Kei
Inagaki, Fumio
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This Document is Protected by copyright and was first published by Frontiers. All rights reserved. it is reproduced with permission. |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
submarine mud volcano, deep subseafloor biosphere |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
methane |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Atribacteria |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
digital PCR |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CARD-FISH |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Submarine mud volcanoes (SMVs) are formed by muddy sediments and breccias extruded to the seafloor from a source in the deep subseafloor and are characterized by the discharge of methane and other hydrocarbon gasses and deep-sourced fluids into the overlying seawater. Although SMVs act as a natural pipeline connecting the Earth’s surface and subsurface biospheres, the dispersal of deep-biosphere microorganisms and their ecological roles remain largely unknown. In this study, we investigated the microbial communities in sediment and overlying seawater at two SMVs located on the Ryukyu Trench off Tanegashima Island, southern Japan. The microbial communities in mud volcano sediments were generally distinct from those in the overlying seawaters and in the well-stratified Pacific margin sediments collected at the Peru Margin, the Juan de Fuca Ridge flank off Oregon, and offshore of Shimokita Peninsula, northeastern Japan. Nevertheless, in-depth analysis of different taxonomic groups at the sub-species level revealed that the taxon affiliated with Atribacteria, heterotrophic anaerobic bacteria that typically occur in organic-rich anoxic subseafloor sediments, were commonly found not only in SMV sediments but also in the overlying seawater. We designed a new oligonucleotide probe for detecting Atribacteria using the catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). CARD-FISH, digital PCR and sequencing analysis of 16S rRNA genes consistently showed that Atribacteria are abundant in the methane plumes of the two SMVs (0.58 and 1.5 × 104 cells/mL, respectively) but not in surrounding waters, suggesting that microbial cells in subseafloor sediments are dispersed as “deep-biosphere seeds” into the ocean. These findings may have important implications for the microbial transmigration between the deep subseafloor biosphere and the hydrosphere. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
| 出版者 |
|
|
出版者 |
Frontiers Media |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 識別子 |
|
|
識別子 |
http://hdl.handle.net/20.500.12000/37630 |
|
識別子タイプ |
HDL |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3389/fmicb.2017.01135 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.3389/fmicb.2017.01135 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1664-302X |
| 収録物名 |
|
|
収録物名 |
Frontiers in Microbiology |
|
言語 |
en |
| 書誌情報 |
巻 8,
p. 1135,
発行日 2017-06-20
|
fmicb-08-01135.pdf
