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  1. 学術雑誌論文
  2. その他
  1. 部局別インデックス
  2. 医学部

Environmental DNA metabarcoding to detect pathogenic Leptospira and associated organisms in leptospirosis-endemic areas of Japan

http://hdl.handle.net/20.500.12000/46981
http://hdl.handle.net/20.500.12000/46981
ff8b06ad-ae24-44c9-a0e1-390c1c19ca4a
名前 / ファイル ライセンス アクション
s41598-019-42978-1.pdf s41598-019-42978-1.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2020-10-14
タイトル
タイトル Environmental DNA metabarcoding to detect pathogenic Leptospira and associated organisms in leptospirosis-endemic areas of Japan
言語 en
作成者 Sato, Yukuto

× Sato, Yukuto

en Sato, Yukuto

Mizuyama, Masaru

× Mizuyama, Masaru

en Mizuyama, Masaru

Sato, Megumi

× Sato, Megumi

en Sato, Megumi

Minamoto, Toshifumi

× Minamoto, Toshifumi

en Minamoto, Toshifumi

Kimura, Ryosuke

× Kimura, Ryosuke

en Kimura, Ryosuke

Toma, Claudia

× Toma, Claudia

en Toma, Claudia

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 en
権利情報 Creative Commons Attribution 4.0
言語 en
権利情報Resource https://creativecommons.org/licenses/by/4.0/
権利情報 https://creativecommons.org/licenses/by/4.0/
内容記述
内容記述タイプ Other
内容記述 Leptospires, which cause the zoonotic disease leptospirosis, persist in soil and aqueous environments. Several factors, including rainfall, the presence of reservoir animals, and various abiotic and biotic components interact to influence leptospiral survival, persistence, and pathogenicity in the environment. However, how these factors modulate the risk of infection is poorly understood. Here we developed an approach using environmental DNA (eDNA) metabarcoding for detecting the microbiome, vertebrates, and pathogenic Leptospira in aquatic samples. Specifically, we combined 4 sets of primers to generate PCR products for high-throughput sequencing of multiple amplicons through next-generation sequencing. Using our method to analyze the eDNA of leptospirosis-endemic areas in northern Okinawa, Japan, we found that the microbiota in each river shifted over time. Operating taxonomic units corresponding to pathogenic L. alstonii, L. kmetyi, and L. interrogans were detected in association with 12 nonpathogenic bacterial species. In addition, the frequencies of 11 of these species correlated with the amount of rainfall. Furthermore, 10 vertebrate species, including Sus scrofa, Pteropus dasymallus, and Cynops ensicauda, showed high correlation with leptospiral eDNA detection. Our eDNA metabarcoding method is a powerful tool for understanding the environmental phase of Leptospira and predicting human infection risk.
内容記述タイプ Other
内容記述 論文
出版者
言語 en
出版者 Springer Nature
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/46981
識別子タイプ HDL
関連情報
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1038/s41598-019-42978-1
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1038/s41598-019-42978-1
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 2045-2322
収録物名
言語 en
収録物名 Scientific Reports
書誌情報
巻 9, 発行日 2019-04-25
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