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  1. 学術雑誌論文
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  2. 医学部

Study on existence of the fisheries resources abundance by using environmental deoxyribonucleic acid (e-DNA) approach at fishing grounds in the Sulawesi Sea, Indonesia

http://hdl.handle.net/20.500.12000/47015
http://hdl.handle.net/20.500.12000/47015
1e6ef5fa-d9ad-4ee2-8073-15d33613b3a7
名前 / ファイル ライセンス アクション
A_Masengi_2019_IOP_Conf._Ser.__Mater._Sci._Eng._567_012026.pdf A_Masengi_2019_IOP_Conf._Ser.__Mater._Sci._Eng._567_012026.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2020-10-20
タイトル
タイトル Study on existence of the fisheries resources abundance by using environmental deoxyribonucleic acid (e-DNA) approach at fishing grounds in the Sulawesi Sea, Indonesia
言語 en
作成者 Masengi, Kawilarang W A

× Masengi, Kawilarang W A

en Masengi, Kawilarang W A

Mandagi, I F

× Mandagi, I F

en Mandagi, I F

Manu, L

× Manu, L

en Manu, L

Silooy, F

× Silooy, F

en Silooy, F

Labaro, I L

× Labaro, I L

en Labaro, I L

Masengi, A W R

× Masengi, A W R

en Masengi, A W R

Sebua, N

× Sebua, N

en Sebua, N

Masengi, E I K G

× Masengi, E I K G

en Masengi, E I K G

Pinontoan, Benny

× Pinontoan, Benny

en Pinontoan, Benny

Hutabarat, Y

× Hutabarat, Y

en Hutabarat, Y

Hukom, F

× Hukom, F

en Hukom, F

Iwata, M

× Iwata, M

en Iwata, M

Abe, Y

× Abe, Y

en Abe, Y

Sato, Y

× Sato, Y

en Sato, Y

Kimura, R

× Kimura, R

en Kimura, R

Yamahira, K

× Yamahira, K

en Yamahira, K

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 en
権利情報 Creative Commons Attribution 3.0
言語 en
権利情報Resource https://creativecommons.org/licenses/by/3.0/
権利情報 https://creativecommons.org/licenses/by/3.0/
内容記述
内容記述タイプ Other
内容記述 Here, we report the results of our preliminary study on deep sea eDNA at fishing ground to approach the fisheries resources abundance at Sulawesi sea by using deep-sea water sampling collected from 10 sites ranging from 110m-200m in depth at front side of the International Coelacanth Research Center and Museum Base at Lolak Waters and Manado Bay North of Sulawesi using Nansen Bottle Sampler (1500 cc). The collected waters were filtered using Power Water Sterivex DNA Isolation Kits and preserved with the DNAiso Reagent then transported to Center for Strategy Research Project, University of the Ryukyus, Okinawa Japan where eDNA analyses were conducted. Our results revealed that the concentrations of eDNA has a good quality were measured with a NanoDrop Lite spectrophotometer, indicating eDNA was successfully extracted. Therefore, by using universal primers for eDNA, MiFish-U-F/R for the 1st-PCR (mt-12S amplification) and 2nd-PCR (tag-indexing) for library preparation to accommodate sequence variations and show that intense signal of MiFish eDNA amplification. Using a high-throughput Illumina MiSeq platform for sequencing analyses, we detected eDNA from 40 fish's species with dominantly by Caranx sexfasciatus, Encrasicholina punctifer.
内容記述タイプ Other
内容記述 論文
出版者
言語 en
出版者 IOP Publishing
言語
言語 eng
資源タイプ
資源タイプ conference paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_5794
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/47015
識別子タイプ HDL
関連情報
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1088/1757-899X/567/1/012026
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1088/1757-899X/567/1/012026
収録物名
言語 en
収録物名 IOP Conference Series: Materials Science and Engineering
書誌情報
巻 567
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