Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2020-10-20 |
タイトル |
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タイトル |
Study on existence of the fisheries resources abundance by using environmental deoxyribonucleic acid (e-DNA) approach at fishing grounds in the Sulawesi Sea, Indonesia |
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言語 |
en |
作成者 |
Masengi, Kawilarang W A
Mandagi, I F
Manu, L
Silooy, F
Labaro, I L
Masengi, A W R
Sebua, N
Masengi, E I K G
Pinontoan, Benny
Hutabarat, Y
Hukom, F
Iwata, M
Abe, Y
Sato, Y
Kimura, R
Yamahira, K
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
en |
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権利情報 |
Creative Commons Attribution 3.0 |
権利情報 |
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言語 |
en |
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権利情報Resource |
https://creativecommons.org/licenses/by/3.0/ |
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権利情報 |
https://creativecommons.org/licenses/by/3.0/ |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Here, we report the results of our preliminary study on deep sea eDNA at fishing ground to approach the fisheries resources abundance at Sulawesi sea by using deep-sea water sampling collected from 10 sites ranging from 110m-200m in depth at front side of the International Coelacanth Research Center and Museum Base at Lolak Waters and Manado Bay North of Sulawesi using Nansen Bottle Sampler (1500 cc). The collected waters were filtered using Power Water Sterivex DNA Isolation Kits and preserved with the DNAiso Reagent then transported to Center for Strategy Research Project, University of the Ryukyus, Okinawa Japan where eDNA analyses were conducted. Our results revealed that the concentrations of eDNA has a good quality were measured with a NanoDrop Lite spectrophotometer, indicating eDNA was successfully extracted. Therefore, by using universal primers for eDNA, MiFish-U-F/R for the 1st-PCR (mt-12S amplification) and 2nd-PCR (tag-indexing) for library preparation to accommodate sequence variations and show that intense signal of MiFish eDNA amplification. Using a high-throughput Illumina MiSeq platform for sequencing analyses, we detected eDNA from 40 fish's species with dominantly by Caranx sexfasciatus, Encrasicholina punctifer. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
IOP Publishing |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
conference paper |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_5794 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/47015 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1088/1757-899X/567/1/012026 |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1088/1757-899X/567/1/012026 |
収録物名 |
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言語 |
en |
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収録物名 |
IOP Conference Series: Materials Science and Engineering |
書誌情報 |
巻 567,
発行日 2019
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