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  1. 学術雑誌論文
  2. その他
  1. 部局別インデックス
  2. 医学部

Understanding leptospirosis eco-epidemiology by environmental DNA metabarcoding of irrigation water from two agro-ecological regions of Sri Lanka

http://hdl.handle.net/20.500.12000/47020
http://hdl.handle.net/20.500.12000/47020
a959bc89-6f85-46fa-ac31-06f7a404de40
名前 / ファイル ライセンス アクション
journal.pntd.0008437.pdf journal.pntd.0008437.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2020-10-20
タイトル
タイトル Understanding leptospirosis eco-epidemiology by environmental DNA metabarcoding of irrigation water from two agro-ecological regions of Sri Lanka
言語 en
作成者 Gamage, Chandika D.

× Gamage, Chandika D.

en Gamage, Chandika D.

Sato, Yukuto

× Sato, Yukuto

en Sato, Yukuto

Kimura, Ryosuke

× Kimura, Ryosuke

en Kimura, Ryosuke

Yamashiro, Tetsu

× Yamashiro, Tetsu

en Yamashiro, Tetsu

Toma, Claudia

× Toma, Claudia

en Toma, Claudia

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 en
権利情報 Creative Commons Attribution 4.0
言語 en
権利情報Resource https://creativecommons.org/licenses/by/4.0/
権利情報 https://creativecommons.org/licenses/by/4.0/
内容記述
内容記述タイプ Other
内容記述 Background:Leptospirosis is one of the most significant zoonoses across the world not only because of its impact on human and animal health but also because of the economic and social impact on agrarian communities. Leptospirosis is endemic in Sri Lanka where paddy farming activities, the use of draught animals in agriculture, and peridomestic animals in urban and rural areas play important roles in maintaining the infection cycle of pathogenic Leptospira, especially concerning animals as a potential reservoir. In this study, an environmental DNA (eDNA) metabarcoding methodology was applied in two different agro-ecological regions of Sri Lanka to understand the eco-epidemiology of leptospirosis.\nMethodology/Principal findings:Irrigation water samples were collected in Kandy District (wet zone mid-country region 2) and Girandurukotte, Badulla District (intermediate zone low-country region 2); and analysed for the presence of pathogenic Leptospira, associated microbiome and the potential reservoir animals. Briefly, we generated PCR products for high-throughput sequencing of multiple amplicons through next-generation sequencing. The analysis of eDNA showed different environmental microbiomes in both regions and a higher diversity of Leptospira species circulating in Kandy than in Girandurukotte. Moreover, the number of sequence reads of pathogenic Leptospira species associated with clinical cases such as L. interrogans was higher in Kandy than in Girandurukotte. Kandy also showed more animal species associated with pathogenic bacterial species than Girandurukotte. Finally, several pathogenic bacterial species including Arcobacter cryaerophilus, responsible for abortion in animals, was shown to be associated with pathogenic Leptospira.\nConclusions/Significance:Leptospirosis has been considered to be endemic in wet regions, consistently, leptospiral sequences were detected strongly in Kandy. The great Leptospira species diversity in Kandy observed in this study shows that the etiological agents of leptospirosis in Sri Lanka might be underestimated. Furthermore, our eDNA metabarcoding can be used to discriminate bacterial and animal species diversity in different regions and to explore environmental microbiomes to identify other associated bacterial pathogens in the environment.
内容記述タイプ Other
内容記述 論文
出版者
言語 en
出版者 Public Library of Science
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/47020
識別子タイプ HDL
関連情報
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1371/journal.pntd.0008437
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1371/journal.pntd.0008437
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1935-2735
収録物識別子タイプ ISSN
収録物識別子 1935-2727
収録物名
言語 en
収録物名 PLoS Neglected Tropical Diseases
書誌情報
巻 14, 号 7
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