| Item type |
デフォルトアイテムタイプ（フル）(1) |
| 公開日 |
2020-11-17 |
| タイトル |
|
|
タイトル |
Genetic diversity, paraphyly and incomplete lineage sorting of mtDNA, ITS2 and microsatellite flanking region in closely related Heliopora species (Octocorallia) |
|
言語 |
en |
| 作成者 |
Yasuda, Nina
Taquet, Coralie
Nagai, Satoshi
Fortes, Miguel
Fan, Tung-Yung
Harii, Saki
Yoshida, Terutoyo
Sito, Yuta
Nadaoka, Kazuo
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ITS2 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Concerted |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
evolution |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
mtMutS |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Secondary structure |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Species identification |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Speciation |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Examining genetic diversity and lineage sorting of different genes in closely related species provide useful information for phylogenetic analyses and ultimately for understanding the origins of biodiversity. In this study, we examined inter- and intraspecific genetic variation in internal transcribed spacer 2 (ITS2), partial mitochondrial gene (mtMutS), and nuclear microsatellite flanking region in two closely related octocoral species (Heliopora coerulea, HC-A and HC-B). These species were recently identified in a population genetic study using microsatellite markers. The two species have different reproductive timing, which ecologically promotes lineage sorting. In this study, we examined whether species boundaries could be detected by the commonly used nuclear ITS2 and mtMutS, as well as by possibly neutral microsatellite flanking sequences. Haplotype network analysis of microsatellite flanking region revealed that a possible ancestral haplotype was still shared between the two species, indicating on-going lineage sorting. Haplotype network analysis of ITS2 and microsatellite flanking region revealed shared haplotypes between the two lineages. The two species shared fewer ITS2 sequences than microsatellite flanking region sequences. The almost fixed point mutation at the tip of helix 3 of ITS2 was not associated with the secondary structure or compensatory base changes (CBCs). The phylogenetic tree of ITS2 showed paraphyly and that of the microsatellite flanking region indicated that lineage sorting for the two species may be incomplete. Much higher intra- and inter-individual variation of ITS2 was observed in HC-B than that in HC-A, highlighting the importance of examining ITS2 from multiple individuals to estimate genetic diversity. The mitochondrial mtMutS gene sequences from 39 individuals, including both species collected from Japan and Taiwan, showed no variation because of slow rates of mitochondrial nucleotide substitution. This study suggests caution is warranted when reciprocal monophyly in a phylogenetic tree is used as the criterion for delimiting closely related octocoral species based on ITS2 or mtMtuS sequences. Detection of boundaries between closely related species requires multi-locus analysis, such as genetic admixture analysis using multiple individuals. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 識別子 |
|
|
識別子 |
http://hdl.handle.net/20.500.12000/47246 |
|
識別子タイプ |
HDL |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.ympev.2015.07.009 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.ympev.2015.07.009 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1055-7903 |
| 収録物名 |
|
|
収録物名 |
Molecular Phylogenetics and Evolution |
|
言語 |
en |
| 書誌情報 |
巻 93,
p. 161-171,
発行日 2015-12
|
1-s2.0-S1055790315002134-main.pdf
