Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2020-11-17 |
タイトル |
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タイトル |
Possible involvement of Tachylectin-2-like lectin from Acropora tenuis in the process of Symbiodinium acquisition |
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言語 |
en |
作成者 |
Kuniya, Nami
Jimbo, Mitsuru
Tanimoto, Fumika
Yamashita, Hiroshi
Koike, Kazuhiko
Harii, Saki
Nakano, Yoshikatsu
Iwao, Kenji
Yasumoto, Ko
Watabe, Shugo
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
en |
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権利情報 |
Creative Commons Attribution 4.0 |
権利情報 |
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言語 |
en |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
N-acetyl-D-galactosamine |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Acropora tenuis |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Coral |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Lectin |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Symbiodinium |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Symbiosis |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Most reef-building corals in tropical and subtropical areas symbiose with microalgae from the genus Symbiodinium (dinoflagellate) and depend on the photosynthate produced by the microalgae. The majority of corals acquire Symbiodinium from the surrounding environment through horizontal transfer, but the molecular mechanisms involved in the acquisition of Symbiodinium remain unknown. It has been hypothesized that carbohydrate-binding proteins, or lectins, of the host coral recognize cell surface carbohydrates of Symbiodinium in the process of acquiring symbionts. Thus, we examined the molecular mechanisms involving lectins and carbohydrates using model organism Acropora tenuis, a common reef-building coral, and Symbiodinium culture strains. Juvenile polyps acquire more cells of Symbiodinium strain NBRC102920 at 72–96 h of metamorphosis induction than in any other period. Glycosidase treatment of Symbiodinium inhibited the acquisition of Symbiodinium by juvenile coral polyps. The presence of carbohydrates D-galactose, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine at 10 mM also tended to decrease Symbiodinium acquisition. We isolated two N-acetyl-D-galactosamine binding lectins with apparent molecular masses of 14.6 and 29.0 kDa from A. tenuis, and de novo sequencing and cDNA cloning showed that the 29.0 kDa protein is Tachylectin-2-like lectin (AtTL-2). The anti-Tachylectin-2 antibody is suggested to bind specifically to AtTL-2. The antibody also inhibited binding of AtTL-2 to N-acetyl-D-galactosamine-resin and the acquisition of Symbiodinium by juvenile A. tenuis polyps. Based on these results, AtTL-2 is likely involved in the process of Symbiodinium acquisition. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
Springer Nature |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/47251 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1007/s12562-015-0862-y |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1007/s12562-015-0862-y |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1444-2906 |
収録物名 |
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言語 |
en |
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収録物名 |
Fisheries Science |
書誌情報 |
巻 81,
号 3,
p. 473-483,
発行日 2015-03-18
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