{"_buckets": {"deposit": "773f2707-105d-4fd3-8af8-7f940dd13ba0"}, "_deposit": {"id": "2011307", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2011307"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02011307", "sets": ["1642838338003", "1642838407795"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Toma, Claudia", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Murray, Gerald L.", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nohara, Toshitsugu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Mizuyama, Masaru", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Koizumi, Nobuo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Adler, Ben", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Suzuki, Toshihiko", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Leptospira interrogans is responsible for the zoonotic disease leptospirosis. The pathogenic mechanisms of this spirochaete remain poorly understood; however, virulence has been correlated with increased phagocytic uptake and survival within macrophages. Leptospiral outer membrane proteins are thought to be responsible for persistence in vivo via interaction with specific host components. In this study, we analysed the transcriptional profile of a virulent strain and its culture\u2010attenuated derivative strain to identify bacterial factors that may be involved in pathogenesis. Two outer membrane proteins, LMB216 and LigB (leptospiral immunoglobulin\u2010like protein B) were downregulated more than 10\u2010fold in the culture\u2010attenuated strain. We show that both proteins play a role in leptospiral uptake by macrophages and that LMB216, as well as LigB, enhances the binding of leptospires to fibronectin. Taken together, our results indicate that LMB216 plays a role in pathogen interaction with host molecule/s, which may contribute to pathogenesis of leptospirosis.", "subitem_description_type": "Other"}, {"subitem_description": "\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "en", "subitem_1522300316516": "Wiley"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/47485"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1462-5822"}, {"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1462-5814"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "Cellular Microbiology"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "9", "bibliographicPageEnd": "1377", "bibliographicPageStart": "1366", "bibliographicVolumeNumber": "16"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "AM", "subitem_1600292170262": "http://purl.org/coar/version/c_ab4af688f83e57aa"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1111/cmi.12296"}}, {"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1111/cmi.12296"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "Toma et al. 2014_ for UR.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2011307/files/Toma et al. 2014_ for UR.pdf"}, "version_id": "161864a0-145c-4f43-b585-559ffe320103"}]}, "item_title": "Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages", "item_type_id": "15", "owner": "1", "path": ["1642838338003", "1642838407795"], "permalink_uri": "http://hdl.handle.net/20.500.12000/47485", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2020-12-15"}, "publish_date": "2020-12-15", "publish_status": "0", "recid": "2011307", "relation": {}, "relation_version_is_last": true, "title": ["Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages"], "weko_shared_id": -1}