{"_buckets": {"deposit": "663adcb0-f4c3-485a-8d32-b44f5727b2b8"}, "_deposit": {"id": "2011538", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2011538"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02011538", "sets": ["1642838338003", "1642838407795"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Murayama, Rie", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kimura-Asami, Mariko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Togo-Ohno, Marina", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yamasaki-Kato, Yumiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Naruse, Taeko K.", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Takeshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hayashi, Takeharu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ai, Tomohiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Spoonamore, Katherine G.", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kovacs, Richard J.", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Vatta, Matteo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Iizuka, Mai", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Saito, Masumi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Wani, Shotaro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hiraoka, Yuichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kimura, Akinori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kuroyanagi, Hidehito", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "en", "subitem_1522651041219": "Creative Commons Attribution 4.0"}, {"subitem_1522650717957": "ja", "subitem_1522651041219": "\u00a9 The Author(s) 2018"}, {"subitem_1522650717957": "en", "subitem_1522650727486": "http://creativecommons.org/licenses/by/4.0/", "subitem_1522651041219": "http://creativecommons.org/licenses/by/4.0/"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "RBM20 is a major regulator of heart-specific alternative pre-mRNA splicing of TTN encoding a giant sarcomeric protein titin. Mutation in RBM20 is linked to autosomal-dominant familial dilated cardiomyopathy (DCM), yet most of the RBM20 missense mutations in familial and sporadic cases were mapped to an RSRSP stretch in an arginine/serine-rich region of which function remains unknown. In the present study, we identified an R634W missense mutation within the stretch and a G1031X nonsense mutation in cohorts of DCM patients. We demonstrate that the two serine residues in the RSRSP stretch are constitutively phosphorylated and mutations in the stretch disturb nuclear localization of RBM20. Rbm20^\u003cS637A\u003e knock-in mouse mimicking an S635A mutation reported in a familial case showed a remarkable effect on titin isoform expression like in a patient carrying the mutation. These results revealed the function of the RSRSP stretch as a critical part of a nuclear localization signal and offer the Rbm20^\u003cS637A\u003e mouse as a good model for in vivo study.", "subitem_description_type": "Other"}, {"subitem_description": "\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "en", "subitem_1522300316516": "Springer Nature"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/48366"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "2045-2322"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "Scientific Reports"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-07-12", "bibliographicIssueDateType": "Issued"}, "bibliographicVolumeNumber": "8"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1038/s41598-018-26624-w"}}, {"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1038/s41598-018-26624-w"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "s41598-018-26624-w.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2011538/files/s41598-018-26624-w.pdf"}, "version_id": "5b3e00bf-c9f1-4d95-8cc6-ec90f70d4cc5"}]}, "item_title": "Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization", "item_type_id": "15", "owner": "1", "path": ["1642838338003", "1642838407795"], "permalink_uri": "http://hdl.handle.net/20.500.12000/48366", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2021-04-27"}, "publish_date": "2021-04-27", "publish_status": "0", "recid": "2011538", "relation": {}, "relation_version_is_last": true, "title": ["Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization"], "weko_shared_id": -1}