Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2020-04-15 |
タイトル |
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タイトル |
Induction of Cell Death in Growing Human T-Cells and Cell Survival in Resting Cells in Response to the Human T-Cell Leukemia Virus Type 1 Tax |
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言語 |
en |
作成者 |
Mizuguchi, Mariko
Sasaki, Yuka
Hara, Toshifumi
Higuchi, Masaya
Tanaka, Yuetsu
Funato, Noriko
Tanaka, Nobuyuki
Fujii, Masahiro
Nakamura, Masataka
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
en |
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権利情報 |
Creative Commons Attribution License 4.0 |
権利情報 |
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言語 |
en |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Tax1 encoded by the human T-cell leukemia virus type 1 (HTLV-1) has been believed todysregulate the expression of cellular genes involved in cell survival and mortality, leadingto the development of adult T-cell leukemia (ATL). The function of Tax1 in ATL developmenthowever is still controversial, primarily because Tax1 induces cell cycle progression andapoptosis. To systemically understand cell growth phase-dependent induction of cell survival or cell death by Tax1, we established a single experimental system using an interleukin 2 (IL-2)-dependent human T-cell line Kit 225 that can be forced into resting phase by IL2 deprivation. Introduction of Tax1 and HTLV-2 Tax (Tax2B) decreased mitochondrial activity alongside apoptosis in growing cells but not in resting cells. Cell cycle profile analysisindicated that Tax1 and Tax2B were likely to perturb the S phase in growing cells. Studieswith Tax1 mutants and siRNA for NF-κB/RelA revealed that Tax1-mediated cell growth inhibition and apoptosis in growing Kit 225 cells depend on RelA. Interestingly, inactivation ofthe non-canonical NF-κB and p38 MAPK pathways relieved Tax1-mediated apoptosis, suggesting that the Tax1-NF-κB-p38 MAPK axis may be associated with apoptosis in growingcells. Inflammatory mediators such as CCL3 and CCL4, which are involved in oncogeneinduced senescence (OIS), were induced by Tax1 and Tax2B in growing cells. In contrast,RelA silencing in resting cells reduced mitochondrial activity, indicating that NF-κB/RelA isalso critical for Tax1-mediated cell survival. These findings suggest that Tax1-mediated cellsurvival and death depend on the cell growth phase. Both effects of Tax1 may be implicatedin the long latency of HTLV-1 infection. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
Public Library of Science |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/45637 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0148217 |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0148217 |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1932-6203 |
収録物名 |
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言語 |
en |
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収録物名 |
PLOS ONE |
書誌情報 |
巻 11,
号 2,
発行日 2016-02-01
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