| Item type |
デフォルトアイテムタイプ（フル）(1) |
| 公開日 |
2020-06-02 |
| タイトル |
|
|
タイトル |
MicroRNA-196a-5p is a potential prognostic marker of delayed lymph node metastasis in early-stage tongue squamous cell carcinoma |
|
言語 |
en |
| 作成者 |
Maruyama, Tessho
Nishihara, Kazuhide
Umikawa, Masato
Arasaki, Akira
Nakasone, Toshiyuki
Nimura, Fumikazu
Matayoshi, Akira
Takei, Kimiko
Nakachi, Saori
Kariya, Ken-Ichi
Yoshimi, Naoki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microRNA |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
tongue |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
oral cavity |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
squamous cell carcinoma |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
miR-196a |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
lymph node metastasis |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
regional recurrence |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
prognostic marker |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
MicroRNAs (miRs) are expected to serve as prognostic tools for cancer. However, many miRs have been reported as prognostic markers of recurrence or metastasis in oral squamous cell carcinoma patients. We aimed to determine the prognostic markers in early‑stage tongue squamous cell carcinoma (TSCC). Based on previous studies, we hypothesized that miR‑10a, 10b, 196a‑5p, 196a‑3p, and 196b were prognostic markers and we retrospectively performed miR expression analyses using formalin‑fixed paraffin‑embedded sections of surgical specimens. Total RNA was isolated from cancer tissues and adjacent normal tissue as control, and samples were collected by laser‑capture microdissection. After cDNA synthesis, reverse transcription‑quantitative polymerase chain reaction was performed. Statistical analyses for patient clinicopathological characteristics, recurrence/metastasis, and survival rates were performed to discern their relationships with miR expression levels, and the 2‑ΔΔCq method was used. miR‑196a‑5p levels were significantly upregulated in early‑stage TSCC, particularly in the lymph node metastasis (LNM) group. The LNM‑free survival rate in the low miR‑196a‑5p ΔΔCq value regulation group was found to be lower than that in the high ΔΔCq value regulation group (P=0.0079). Receiver operating characteristic analysis of ΔΔCq values revealed that miR‑196a‑5p had a P‑value=0.0025, area under the curve=0.740, and a cut‑off value=‑0.875 for distinguishing LNM. To our knowledge, this is the first study to examine LNM‑related miRs in early‑stage TSCC as well as miRs and ‘delayed LNM’ in head and neck cancer. miR‑196a‑5p upregulation may predict delayed LNM. Our data serve as a foundation for future studies to evaluate miR levels and facilitate the prediction of delayed LNM during early‑stage TSCC, which prevent metastasis when combined with close follow‑up and aggressive adjuvant therapy or elective neck dissection. Moreover, our data will serve as a foundation for future studies to evaluate whether miR‑196a‑5p can serve as a therapeutic marker for preventing metastasis. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
| 出版者 |
|
|
出版者 |
Spandidos Publications |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 識別子 |
|
|
識別子 |
http://hdl.handle.net/20.500.12000/45982 |
|
識別子タイプ |
HDL |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3892/ol.2017.7562 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3892/ol.2017.7562 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1792-1074 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1792-1082 |
| 収録物名 |
|
|
収録物名 |
Oncology Letters |
|
言語 |
en |
| 書誌情報 |
巻 15,
号 2,
p. 2349-2363,
発行日 2018-01
|
ol_15_2_2349_PDF.pdf
