Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2020-06-03 |
タイトル |
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タイトル |
Distinct gene expression signatures induced by viral transactivators of different HTLV-1 subgroups that confer a different risk of HAM/TSP |
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言語 |
en |
作成者 |
Naito, Tadasuke
Yasunaga, Jun-ichirou
Mitobe, Yuichi
Shirai, Kazumasa
Sejima, Hiroe
Ushirogawa, Hiroshi
Tanaka, Yuetsu
Nakamura, Tatsufumi
Hanada, Kousuke
Fujii, Masahiro
Matsuoka, Masao
Saito, Mineki
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
en |
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権利情報 |
Creative Commons Attribution 4.0 |
権利情報 |
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言語 |
en |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
HTLV-1 |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Virus subgroup |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
HAM/TSP |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Tax |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
HBZ |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Microarray |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Background: Among human T cell leukemia virus type 1 (HTLV-1)-infected individuals, there is an association between HTLV-1 tax subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population. To investigate the role of HTLV-1 subgroups in viral pathogenesis, we studied the functional diference in the subgroupspecifc viral transcriptional regulators Tax and HBZ using microarray analysis, reporter gene assays, and evaluation of viral-host protein–protein interaction. Results: (1) Transcriptional changes in Jurkat Tet-On human T-cells that express each subgroup of Tax or HBZ protein under the control of an inducible promoter revealed diferent target gene profles; (2) the number of diferentially regulated genes induced by HBZ was 2–3 times higher than that induced by Tax; (3) Tax and HBZ induced the expres‑ sion of diferent classes of non-coding RNAs (ncRNAs); (4) the chemokine CXCL10, which has been proposed as a prognostic biomarker for HAM/TSP, was more efciently induced by subgroup-A Tax (Tax-A) than subgroup-B Tax (Tax-B), in vitro as well as in unmanipulated (ex vivo) PBMCs obtained from HAM/TSP patients; (5) reporter gene assays indicated that although transient Tax expression in an HTLV-1-negative human T-cell line activated the CXCL10 gene promoter through the NF-κB pathway, there was no diference in the ability of each subgroup of Tax to activate the CXCL10 promoter; however, (6) chromatin immunoprecipitation assays showed that the ternary complex containing Tax-A is more efciently recruited onto the promoter region of CXCL10, which contains two NF-κB binding sites, than that containing Tax-B. Conclusions: Our results indicate that diferent HTLV-1 subgroups are characterized by diferent patterns of host gene expression. Diferential expression of pathogenesis-related genes by subgroup-specifc Tax or HBZ may be asso‑ ciated with the onset of HAM/TSP. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
BioMed Central |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/46016 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1186/s12977-018-0454-x |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1186/s12977-018-0454-x |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1742-4690 |
収録物名 |
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言語 |
en |
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収録物名 |
Retrovirology |
書誌情報 |
巻 15,
発行日 2018-11-06
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