Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2020-06-03 |
タイトル |
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タイトル |
Absolute quantification of HTLV-1 basic leucine zipper factor (HBZ) protein and its plasma antibody in HTLV-1 infected individuals with different clinical status |
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言語 |
en |
作成者 |
Shiohama, Yasuo
Naito, Tadasuke
Matsuzaki, Toshio
Tanaka, Reiko
Tomoyose, Takeaki
Takashima, Hiroshi
Fukushima, Takuya
Tanaka, Yuetsu
Saito, Mineki
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
en |
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権利情報 |
Creative Commons Attribution 4.0 |
権利情報 |
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言語 |
en |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
HTLV-1 |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
HBZ |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
ATL |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Monoclonal antibody |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
ELISA |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Background: Human T cell leukemia virus type 1 (HTLV-1) basic leucine zipper factor (HBZ), which is encoded by a minus strand mRNA, is thought to play important roles in the development of adult T-cell leukemia (ATL) and HTLV1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). However, a comprehensive analysis of HBZ, including mRNA and protein expression, humoral immunoreactivity against HBZ, and HTLV-1 proviral load (PVL), in HTLV1-infected individuals with different clinical status has not been reported previously. Results: In this study, using novel monoclonal antibody-based in-house enzyme-linked immunosorbent assay systems, we report the absolute quantification of HBZ protein and its plasma antibody in clinical samples from HTLV-1-infected individuals with different clinical status. The data were compared to both HBZ mRNA levels and PVL. The results showed that plasma anti-HBZ antibody was detectable only in 10.4 % (5/48) of asymptomatic carriers (ACs), 10.8 % (13/120) of HAM/TSP patients, and 16.7 % (7/42) of ATL patients. HBZ protein was detected in three out of five patients with acute ATL, but was not detected in patients with HAM/TSP (0/10) or ACs (0/4). Thus, an antibody response to HBZ was not associated with the PVL or the expression of HBZ (both at the mRNA and protein levels) or the clinical status of the infection. Conclusions: The present results emphasize the extremely low expression and immunogenicity of HBZ in natural HTLV-1 infection. However, there is a possibility that the low but distinct expression of HBZ protein in PBMCs is associated with the survival of HTLV-1-infected cells and the development of ATL. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
BioMed Central |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/46015 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1186/s12977-016-0263-z |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1186/s12977-016-0263-z |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1742-4690 |
収録物名 |
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言語 |
en |
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収録物名 |
Retrovirology |
書誌情報 |
巻 13,
発行日 2016-04-27
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