Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2020-08-05 |
タイトル |
|
|
タイトル |
Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone |
|
言語 |
en |
作成者 |
Higa-Nakamine, Sayomi
Maeda, Noriko
Toku, Seikichi
Yamamoto, Hideyuki
|
アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
ErbB4 |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Fyn |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
GnRH |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Protein kinase C |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Protein kinase D |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
The receptor for gonadotropin-releasing hormone (GnRH) belongs to the G protein-coupled receptors (GPCRs), and its stimulation activates extracellular signal-regulated protein kinase (ERK). We found that the transactivation of ErbB4 was involved in GnRH-induced ERK activation in immortalized GnRH neurons (GT1–7 cells). We found also that GnRH induced the cleavage of ErbB4. In the present study, we examined signal transduction for the activation of ERK and the cleavage of ErbB4 after GnRH treatment. Both ERK activation and ErbB4 cleavage were completely inhibited by YM-254890, an inhibitor of G_<q/11> proteins. Down-regulation of protein kinase C (PKC) markedly decreased both ERK activation and ErbB4 cleavage. Experiments with two types of PKC inhibitors, Gö 6976 and bisindolylmaleimide I, indicated that novel PKC isoforms but not conventional PKC isoforms were involved in ERK activation and ErbB4 cleavage. Our experiments indicated that the novel PKC isoforms activated protein kinase D (PKD) after GnRH treatment. Knockdown and inhibitor experiments suggested that PKD1 stimulated the phosphorylation of Pyk2 by constitutively activated Src and Fyn for ERK activation. Taken together, it is highly possible that PKD1 plays a critical role in signal transduction from the PKC pathway to the tyrosine kinase pathway. Activation of the tyrosine kinase pathway may be involved in the progression of cancer. |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
出版者 |
|
|
言語 |
en |
|
出版者 |
American Society for Biochemistry and Molecular Biology |
言語 |
|
|
言語 |
eng |
資源タイプ |
|
|
資源タイプ |
journal article |
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
識別子 |
|
|
識別子 |
http://hdl.handle.net/20.500.12000/46586 |
|
識別子タイプ |
HDL |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://dx.doi.org/10.1074/jbc.M115.681700 |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1074/jbc.M115.681700 |
収録物識別子 |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1083-351X |
収録物識別子 |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
0021-9258 |
収録物名 |
|
|
言語 |
en |
|
収録物名 |
Journal of Biological Chemistry |
書誌情報 |
巻 290,
号 43,
p. 25974-25985,
発行日 2015-10-23
|