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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 26巻3・4号
  1. 部局別インデックス
  2. その他

[原著]Proteomic Changes in a Squamous Cell Carcinoma Cell Line Induced by an Effector Kinase of a Small G Protein Rap 2

http://hdl.handle.net/20.500.12000/0002015567
http://hdl.handle.net/20.500.12000/0002015567
cdec881d-6160-46a1-9cfc-254b14e59107
名前 / ファイル ライセンス アクション
v26p135.pdf v26p135.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2010-02-23
タイトル
タイトル [原著]Proteomic Changes in a Squamous Cell Carcinoma Cell Line Induced by an Effector Kinase of a Small G Protein Rap 2
言語 ja
作成者 Kuninaka, Kouichi

× Kuninaka, Kouichi

en Kuninaka, Kouichi

Yamashiro, Yoshito

× Yamashiro, Yoshito

en Yamashiro, Yoshito

BayarJargal, Maitsetseg

× BayarJargal, Maitsetseg

en BayarJargal, Maitsetseg

Nishimaki, Tadashi

× Nishimaki, Tadashi

en Nishimaki, Tadashi

Kariva, Ken-ichi

× Kariva, Ken-ichi

en Kariva, Ken-ichi

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 Proteomics
言語 en
主題Scheme Other
主題 Rap2
言語 en
主題Scheme Other
主題 MAP4K
言語 en
主題Scheme Other
主題 Squamous cell carcinoma
内容記述
内容記述タイプ Other
内容記述 The Traf2- and Nck-interacting kinase(TNIK) is a downstream effector of a Ras-like small G protein Rap2 identified by us. Very little is known about TNIK's roles in physiological or pathological cellular processes.To examine the effect of activated TNIK in cancer cells, over-expression of TNIK was induced by the use of TetOffsystem in a squamous cell carcinoma cell line. Global protein expression profiling was carried out by Ettan TM twodimensional fluorescence difference gel electrophoresis(2-D DIGE). Proteins were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. By comparlng the cells with or without over-expressed TNIK, 2-D DIGE revealed up-regulation of three proteins and down-regulation of six proteins by TNIK.Peptide mass fingerprinting with MALDI-TOF mass spectrometry identified five out of these nine proteins:isovaleryl coenzyme-A dehydrogenase (up-regulated 2.46-fold, p<0.05); Rho GDP dissociationin hibitor alpha(down-regulated2.35-fold, p<0.05); stomatin-like protein 2 (down-regulated 1.57-fold, p<0.05); ribosomal protein,mitochondrial,S22(down-regulated 1.78-fold,p<0.05); Ornithine aminotransferase (down-regulated 1.34-fold, p<0.05). Three down-regulated proteins, Rho GDP dissociation inhibitor alpha, stomatin1ike protein 2, and ornithine aminotransferase were candidates for cancer prognostic marker or target proteins for chemotheraputic drug development.
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346-888X
収録物識別子タイプ ISSN
収録物識別子 0289-1530
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 26, 号 3・4, p. 135-145
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