{"_buckets": {"deposit": "f78c4633-492c-477a-b160-09dc6b8f2ffc"}, "_deposit": {"id": "2015567", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2015567"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02015567", "sets": ["1642838224252", "1642838412624"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\uff3b\u539f\u8457\uff3dProteomic Changes in a Squamous Cell Carcinoma Cell Line Induced by an Effector Kinase of a Small G Protein Rap 2", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kuninaka, Kouichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yamashiro, Yoshito", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "BayarJargal, Maitsetseg", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nishimaki, Tadashi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kariva, Ken-ichi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "ja", "subitem_1522651041219": "\u7409\u7403\u533b\u5b66\u4f1a"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Proteomics"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Rap2"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "MAP4K"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Squamous cell carcinoma"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "The Traf2- and Nck-interacting kinase(TNIK) is a downstream effector of a Ras-like small G protein Rap2 identified by us. Very little is known about TNIK\u0027s roles in physiological or pathological cellular processes\uff0eTo examine the effect of activated TNIK in cancer cells, over-expression of TNIK was induced by the use of TetOffsystem in a squamous cell carcinoma cell line. Global protein expression profiling was carried out by Ettan TM twodimensional fluorescence difference gel electrophoresis(2-D DIGE). Proteins were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. By comparlng the cells with or without over\uff0dexpressed TNIK, 2-D DIGE revealed up-regulation of three proteins and down-regulation of six proteins by TNIK.Peptide mass fingerprinting with MALDI-TOF mass spectrometry identified  five out of these nine proteins\uff1aisovaleryl coenzyme-A dehydrogenase (up-regulated 2.46-fold, p\u003c0.05); Rho GDP dissociationin hibitor alpha(down-regulated2.35-fold, p\u003c0.05); stomatin-like protein 2 (down-regulated 1.57-fold, p\u003c0.05); ribosomal protein,mitochondrial,S22\uff08down-regulated 1.78-fold,p\u003c0.05); Ornithine aminotransferase (down-regulated 1.34-fold, p\u003c0.05). Three down-regulated proteins, Rho GDP dissociation inhibitor alpha, stomatin1ike protein 2, and ornithine aminotransferase were candidates for cancer prognostic marker or target proteins for chemotheraputic drug development.", "subitem_description_type": "Other"}, {"subitem_description": "\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u533b\u5b66\u4f1a"}, {"subitem_1522300295150": "en", "subitem_1522300316516": "Ryukyu Medical Association"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1346-888X"}, {"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0289-1530"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN10369445"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u533b\u5b66\u4f1a\u8a8c = Ryukyu Medical Journal"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "3\u30fb4", "bibliographicPageEnd": "145", "bibliographicPageStart": "135", "bibliographicVolumeNumber": "26"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "v26p135.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2015567/files/v26p135.pdf"}, "version_id": "6553f855-eca8-4794-8ad0-e1858944b898"}]}, "item_title": "\uff3b\u539f\u8457\uff3dProteomic Changes in a Squamous Cell Carcinoma Cell Line Induced by an Effector Kinase of a Small G Protein Rap 2", "item_type_id": "15", "owner": "1", "path": ["1642838224252", "1642838412624"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002015567", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-02-23"}, "publish_date": "2010-02-23", "publish_status": "0", "recid": "2015567", "relation": {}, "relation_version_is_last": true, "title": ["\uff3b\u539f\u8457\uff3dProteomic Changes in a Squamous Cell Carcinoma Cell Line Induced by an Effector Kinase of a Small G Protein Rap 2"], "weko_shared_id": -1}