Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2010-02-23 |
タイトル |
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タイトル |
[原著]Mechanical Stress regulates Chondrocyte Proliferation and Differentiation during Endochondral Bone Formation |
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言語 |
ja |
作成者 |
Horizono, Hidemro
Owan, Ichiro
Kudoh, Hirohisa
Arakaki, Harumi
Kanaya, Fuminori
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
ja |
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権利情報 |
琉球医学会 |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
mechanical stress |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
chondrocyte |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
chondrogenesis endochondral bone formation |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
The effects of mechanical stress on the proliferation and differentiation of mesenchymal progenitor cells toward chondrogenesis are not well addressed. The purpose of this study was to assess how mechanical stress exerts the influence on chondrogenic proliferation and differentiation during endochondral bone formation. ATDC5 mouse chondroprogenitor cells, which undergo a reproducible multistep chondrogenic differentiation in response to insulin, were used in this study. Mechanical loading was applied to ATDC5 cells grown on plastic plates in monolayer cultures at a magnitude of 2800 $ \mu $strain and a frequency of 0.5 Hz for 24 hr at day 4, 7, 14, 21 and 28. The chondrogenesis pathway m ATDC5 cells was studied by analyzing differentiation stage-specific gene expression using a Northern blot analysis. ATDC5 cells responded to the addition of insulin with a significant increase in the expression of mRNA for histone H4, type II collagen, and aggrecan, but not type X collagen during a 28-day culture period. These inductive responses were time-dependent, and therefore, differentiation-dependent. Applying mechamcal loading in the presence of insulin further up-regulated the mRNA levels of these proteins including type X collagen. Moreover, mechanical loading increased the histone H4 mRNA expression in immature cells, and promoted the mRNA expression of cartilage-type extracellular matrix in insulin-induced relatively mature cells, which also indicated differentiation-dependent. The expression of histone H4, type II and X collagen, and aggrecan mRNA levels were also increased with mechanical loading alone. These results demonstrated the adjunctive effects of mechanical loading on insulininduced chondrogenesis and the peculiar effects of mechanical loading on the induction of chondrogenesis, thus suggesting that mechanical loading can promote endochondral bone formation. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
ja |
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出版者 |
琉球医学会 |
出版者 |
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言語 |
en |
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出版者 |
Ryukyu Medical Association |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346-888X |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0289-1530 |
収録物識別子 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN10369445 |
収録物名 |
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言語 |
ja |
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収録物名 |
琉球医学会誌 = Ryukyu Medical Journal |
書誌情報 |
巻 26,
号 1・2,
p. 57-67,
発行日 2007
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