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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 26巻1・2号
  1. 部局別インデックス
  2. その他

[原著]Mechanical Stress regulates Chondrocyte Proliferation and Differentiation during Endochondral Bone Formation

http://hdl.handle.net/20.500.12000/0002015577
http://hdl.handle.net/20.500.12000/0002015577
b29edd1e-d828-4a51-960e-af35fba014bb
名前 / ファイル ライセンス アクション
v26p57.pdf v26p57.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2010-02-23
タイトル
タイトル [原著]Mechanical Stress regulates Chondrocyte Proliferation and Differentiation during Endochondral Bone Formation
言語 ja
作成者 Horizono, Hidemro

× Horizono, Hidemro

en Horizono, Hidemro

Owan, Ichiro

× Owan, Ichiro

en Owan, Ichiro

Kudoh, Hirohisa

× Kudoh, Hirohisa

en Kudoh, Hirohisa

Arakaki, Harumi

× Arakaki, Harumi

en Arakaki, Harumi

Kanaya, Fuminori

× Kanaya, Fuminori

en Kanaya, Fuminori

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 mechanical stress
言語 en
主題Scheme Other
主題 chondrocyte
言語 en
主題Scheme Other
主題 chondrogenesis endochondral bone formation
内容記述
内容記述タイプ Other
内容記述 The effects of mechanical stress on the proliferation and differentiation of mesenchymal progenitor cells toward chondrogenesis are not well addressed. The purpose of this study was to assess how mechanical stress exerts the influence on chondrogenic proliferation and differentiation during endochondral bone formation. ATDC5 mouse chondroprogenitor cells, which undergo a reproducible multistep chondrogenic differentiation in response to insulin, were used in this study. Mechanical loading was applied to ATDC5 cells grown on plastic plates in monolayer cultures at a magnitude of 2800 $ \mu $strain and a frequency of 0.5 Hz for 24 hr at day 4, 7, 14, 21 and 28. The chondrogenesis pathway m ATDC5 cells was studied by analyzing differentiation stage-specific gene expression using a Northern blot analysis. ATDC5 cells responded to the addition of insulin with a significant increase in the expression of mRNA for histone H4, type II collagen, and aggrecan, but not type X collagen during a 28-day culture period. These inductive responses were time-dependent, and therefore, differentiation-dependent. Applying mechamcal loading in the presence of insulin further up-regulated the mRNA levels of these proteins including type X collagen. Moreover, mechanical loading increased the histone H4 mRNA expression in immature cells, and promoted the mRNA expression of cartilage-type extracellular matrix in insulin-induced relatively mature cells, which also indicated differentiation-dependent. The expression of histone H4, type II and X collagen, and aggrecan mRNA levels were also increased with mechanical loading alone. These results demonstrated the adjunctive effects of mechanical loading on insulininduced chondrogenesis and the peculiar effects of mechanical loading on the induction of chondrogenesis, thus suggesting that mechanical loading can promote endochondral bone formation.
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346-888X
収録物識別子タイプ ISSN
収録物識別子 0289-1530
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 26, 号 1・2, p. 57-67
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