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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 27巻1・2号
  1. 部局別インデックス
  2. その他

[原著]生物活性または発現活性を保持している LINE1 (L1) のゲノムワイド探索

http://hdl.handle.net/20.500.12000/0002015587
http://hdl.handle.net/20.500.12000/0002015587
978a0445-0a89-4375-a770-1bd8cdb1b666
名前 / ファイル ライセンス アクション
v27p11.pdf v27p11.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2010-02-23
タイトル
タイトル [原著]生物活性または発現活性を保持している LINE1 (L1) のゲノムワイド探索
言語 ja
作成者 神山, 聡子

× 神山, 聡子

ja 神山, 聡子

小田, 高也

× 小田, 高也

ja 小田, 高也

陣野, 吉廣

× 陣野, 吉廣

ja 陣野, 吉廣

Kamiyama, Satoko

× Kamiyama, Satoko

en Kamiyama, Satoko

Oda, Takaya

× Oda, Takaya

en Oda, Takaya

Jinno, Yoshihiro

× Jinno, Yoshihiro

en Jinno, Yoshihiro

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 retrotransposon
言語 en
主題Scheme Other
主題 LINE1 (L1)
言語 en
主題Scheme Other
主題 transcriptional potential
言語 en
主題Scheme Other
主題 genome-wide search
内容記述
内容記述タイプ Other
内容記述 One of research subjects in our laboratory is to elucidate roles of DNA methylation and retrotransposons in the pathogenesis of complex hereditary diseases/disorders, for example schizophrenia. So far we have identified several human endogenous retroviruses (HERVs) with transcriptional potential in the brain as well as those abundantly expressed in the placenta. In our current study we performed a genome-wide search for transcriptionally active long interspersed nuclear elements 1 (LINE1s; L1s) We adopted two different ways to find out them beginning the search with human expressed sequence tag (EST) and genome-DNA databases. The full-length Sequence of L1 3 (6,059 bp) was used as a query in the both cases. We collected 5,000 ESTs homologous to L1 3, mapped them to chromosomal L1 loci, and finally extracted 254 loci from which three and more ESTs were derived In the search of genome-DNA databases we found 5,134 L1 sequences homologous to L1 3 and downloaded them together with 1-kb flanking sequences. We finally narrowed down to 146 L1s that retained both of the ORF1- and ORF2-coding amino acid sequences. Some of these L1s were subjected to the first verification experiment using the RT-PCR, cloning, and sequencing method Two or more clones with identical sequences were found more frequently in RT-PCR than in genomic PCR The distribution of L1 subfamilies detected in PCR clones was apparently different between the two kinds of PCR These results suggested that RT-PCR detected some L1 loci different from genomic DNA in their frequency and that our current study should include something worth analyzing in further detail although the development of more efficient techniques is essential to the rapid and extensive progress of the study.
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
言語
言語 jpn
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346-888X
収録物識別子タイプ ISSN
収録物識別子 0289-1530
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 27, 号 1・2, p. 11-18
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