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[原著]Amino Acid Sequence of Avian Hemoglobins and Its Evolutiomary Implication at仙e Molecular Level 1. Partial Amino Acid Sequence of the $ \alpha $ Chain of Japanese Quail QII Hemoglobin
http://hdl.handle.net/20.500.12000/0002015753
http://hdl.handle.net/20.500.12000/0002015753e5815cdf-9c8f-4d5c-9be1-f9c9b8159caf
名前 / ファイル | ライセンス | アクション |
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v7p79.pdf
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Item type | デフォルトアイテムタイプ(フル)(1) | |||||||||
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公開日 | 2010-06-30 | |||||||||
タイトル | ||||||||||
タイトル | [原著]Amino Acid Sequence of Avian Hemoglobins and Its Evolutiomary Implication at仙e Molecular Level 1. Partial Amino Acid Sequence of the $ \alpha $ Chain of Japanese Quail QII Hemoglobin | |||||||||
言語 | ja | |||||||||
作成者 |
Takei, Hiroshi
× Takei, Hiroshi
× Eguchi, Yukinori
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アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
権利情報 | ||||||||||
言語 | ja | |||||||||
権利情報 | 琉球医学会 | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | $ \alpha $ chain | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | amino acid sequence | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | hemoglobin | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Japanese quail | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | evolution | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | Amino acid sequence of the $ \alpha $ chain of Japanese quail (coutournix coutournix japonica) hemoglobin QII was investigated and determined except its 'core' portion. The carboxymethylated $ \alpha $ chain was hydrolysed with trypsin and 13 tryptic peptide fragments were purified by using ion exchange chromatography, Sephadex gel filtration, etc. Ten peptides of them, T-1, T-2, T-3, T-5, T-7, T-8, T-10, T-11, T-12 and T-13 were short ones and their amino acid sequence was directly determined mainly by Edman degradation method. Since peptides T-4 and T-6 were longer ones, they were further hydrolysed with chymotrypsin and the amino acid sequence of the chymotryptic peptides purified was respectively determined by DNS-Edman or Edman degradation method. The peptide T-9 was also a longer one and was subjected to digestion with thermolysin and the amino acid sequence of the thermolytic peptides purified was determined by Edman degradation method. The amino acid sequence of the soluble fraction of the $ \alpha $ chain of Japanese quail QII hemoglobin thus determined was compared to that of chicken All hemoglobin. Their homology was so high with each other that the percentage of amino acid differences was 10.9 and the amino acid residues placed in the important position involved in hemoglobin function was less variable. | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 論文 | |||||||||
出版者 | ||||||||||
言語 | ja | |||||||||
出版者 | 琉球大学医学部 | |||||||||
出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Faculty of Medicine, University of the Ryukyus | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ | journal article | |||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
出版タイプ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 0289-1530 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AN10027430 | |||||||||
収録物名 | ||||||||||
言語 | ja | |||||||||
収録物名 | 琉球大学医学会雑誌 : 医学部紀要 = Ryukyu medical journal | |||||||||
書誌情報 |
巻 7, 号 2, p. 79-89, 発行日 1984 |