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[原著]ENHANCEMENT OF HEPATIC MICROSOMALAMINO・PYRINE N-DEMETHYLATION BY NEUTRAL SALTS
http://hdl.handle.net/20.500.12000/0002015756
http://hdl.handle.net/20.500.12000/00020157562a859082-da34-46e5-8ebb-6566af4e46f8
名前 / ファイル | ライセンス | アクション |
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v9p151.pdf
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Item type | デフォルトアイテムタイプ(フル)(1) | |||||||||
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公開日 | 2010-06-30 | |||||||||
タイトル | ||||||||||
タイトル | [原著]ENHANCEMENT OF HEPATIC MICROSOMALAMINO・PYRINE N-DEMETHYLATION BY NEUTRAL SALTS | |||||||||
言語 | ja | |||||||||
作成者 |
Aniya, Yoko
× Aniya, Yoko
× Matsusaki, Kichihiko
|
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アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
権利情報 | ||||||||||
言語 | ja | |||||||||
権利情報 | 琉球医学会 | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | aminopyrine | |||||||||
主題 | ||||||||||
言語 | ja | |||||||||
主題Scheme | Other | |||||||||
主題 | N-demethylation | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | neutral salt | |||||||||
主題 | ||||||||||
言語 | ja | |||||||||
主題Scheme | Other | |||||||||
主題 | cytochrome P-450 | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | Enhancement of aminopyrine N-demethylation by neutral salts was kinetically studied using hepatic microsomes from nontreated, phenobarbital- or 3-methylcholanthrene-treated rats. Aminopyrine N-demethylation was increased 3-fold by 0.4 M sodium sulfate in nontreated or 3-methylcholanthrene-treated microsomes. On the other hand, the enhancement was 1.7-fold in phenobarbital-treated microsomes. Lineweaver-Burk plots of aminopyrine N-demethylase were not linear in nontreated microsomes and two Km values (2X10^<-3>M, 5X10^<-4>M) were obtained. However, the addition of neutral salts to the microsomes brought on a linear Lineweaver-Burk plots of the demethylation and only one km, which was less than 2X10^<-3>M. Aminopyrine-induced difference spectra of the microsomes were increased by the addition of neutral salts, and double reciprocal plots of the spectrum vs aminopyrine concentration were linear, though the plots were not linear in absence of the salts. Dissociation constant (Ks) values were also decreased by the addition of neutral salts corresponding enhancement of the demethylation, as were the km values. In phenobarbital or 3-methylcholanthrene-treated microsomes one Km was obtained regardless of the presence or absence of sodium sulfate, though Km of the demethylase in the former microsomes was higher than that in the latter after addition of the salt. These results suggest that there are multiple forms of aminopyrine N-demethylase in nontreated and 3-methylcholanthrene -treated microsomes and some of them slightly contribute to the demethylation in normal condition but exhibite the demethylase activity by addition of the salts. Phenobarbital may induce the aminopyrine N-demethylase capable of demethylating aminopyrine in normal condition resulted in less enhancement of the demethylation by the salts. | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 論文 | |||||||||
出版者 | ||||||||||
言語 | ja | |||||||||
出版者 | 琉球大学医学部 | |||||||||
出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Faculty of Medicine, University of the Ryukyus | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ | journal article | |||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
出版タイプ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 0289-1530 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AN10027430 | |||||||||
収録物名 | ||||||||||
言語 | ja | |||||||||
収録物名 | 琉球大学医学会雑誌 : 医学部紀要 = Ryukyu medical journal | |||||||||
書誌情報 |
巻 9, 号 3-4, p. 151-159, 発行日 1986 |