| Item type |
デフォルトアイテムタイプ（フル）(1) |
| 公開日 |
2010-07-02 |
| タイトル |
|
|
タイトル |
[原著］Expression of Japanese Encephalitis Virus Envelope Protein by Baculovirus Expression System for the Analysis of Immunopathogenesis |
|
言語 |
ja |
| 作成者 |
Arakaki, Sakae
Makino, Yoshihiro
Tadano, Masayuki
Fukunaga, Toshihiko
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
琉球医学会 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Japanese encephalitis virus |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
expressed E protein |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
patient sera |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
sequential flavivirus infection |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
secondary immune response |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
We have constructed C-terminally truncated Japanese encephalitis virus envelope (E) proteins that ranged in length from 260 amino acids (a. a.) to 380 a. a. of the N-terminal sequence, using the baculovirus expression system, to analyze antigenic sites and examine basic conditions to keep a proper configuration of E protein. Truncated E proteins longer than 304 a. a. residues reacted with patients sera with sequential flavivirus infection, but failed to react with sera with primary flavivirus, even JE virus, infection. The E proteins of 279 a. a. or shorter residues did not react with sera from both patients with primary and sequential flavivirus infection. These results suggest that the main epitopes recognized during the secondary flavivirus infection are different from those recognized during the primary flavivirus infection, and also that existence of the fifth disulphide bond (a. a. #190 and a. a. #287) is involved in the reactivity of recombinant E proteins with patient sera in sequential flavivirus infection. Analysis of the length of expressed membrane (M) protein combined with E protein revealed that M proteins shorter than 37 a. a. residues failed to react with any of patient sera. This suggests that at least the C-terminal 37 a.a. residues of M protein is needed for the proper cleavage between M and E proteins. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
| 出版者 |
|
|
出版者 |
琉球医学会 |
|
言語 |
ja |
| 出版者 |
|
|
出版者 |
Ryukyu Medical Association |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1346888X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0289-1530 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10369445 |
| 収録物名 |
|
|
収録物名 |
琉球医学会誌 = Ryukyu Medical Journal |
|
言語 |
ja |
| 書誌情報 |
巻 14,
号 1,
p. 25-32,
発行日 1994
|
v14p25.pdf
