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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 17巻2号
  1. 部局別インデックス
  2. その他

[原著]培養単層血管内皮細胞でのアルブミン透過性に対する高濃度グルコースの影響

http://hdl.handle.net/20.500.12000/0002016110
http://hdl.handle.net/20.500.12000/0002016110
9022b896-4383-48dc-909e-13677e9d2f1c
名前 / ファイル ライセンス アクション
v17p89.pdf v17p89.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2010-07-02
タイトル
タイトル [原著]培養単層血管内皮細胞でのアルブミン透過性に対する高濃度グルコースの影響
言語 ja
作成者 平良, 剛

× 平良, 剛

ja 平良, 剛

田仲, 秀明

× 田仲, 秀明

ja 田仲, 秀明

平良, 美香

× 平良, 美香

ja 平良, 美香

新垣, 香代子

× 新垣, 香代子

ja 新垣, 香代子

小宮, 一郎

× 小宮, 一郎

ja 小宮, 一郎

村上, 啓治

× 村上, 啓治

ja 村上, 啓治

Taira, Tsuyoshi

× Taira, Tsuyoshi

en Taira, Tsuyoshi

Tanaka, Hideaki

× Tanaka, Hideaki

en Tanaka, Hideaki

Taira, Mika

× Taira, Mika

en Taira, Mika

Arakaki, Kayoko

× Arakaki, Kayoko

en Arakaki, Kayoko

Komiya, Ichiro

× Komiya, Ichiro

en Komiya, Ichiro

Murakami, Keiji

× Murakami, Keiji

en Murakami, Keiji

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 albumin transcytosis
言語 en
主題Scheme Other
主題 endothelial cell monolayer
言語 en
主題Scheme Other
主題 Evans'blue dye
言語 en
主題Scheme Other
主題 diabetes mellitus
内容記述
内容記述タイプ Other
内容記述 Increased vascular permeability is one of the characteristics of diabetic patients. The endothehal cells regulate the vascular permeability. We studied the vascular permeability using albumin. We intended to make a model of albumin transcytosis, and to reveal the mechanism of glucose-induced albumin transcytosis. Endothelial cells were cultured on collagen-coated microporous filter to make an endothelial cell monolayer on the filter. This monolayer was placed between the upper well and the lower one. To estimate albumin transcytosis from the upper wells (luminal sides) to the lower wells (basolateral sides), we added Evans' blue dye labeled albumin (EBA) in the upper wells and measured the amount of EBA in the lower wells. The amounts of EBA in the lower wells increased with the hours of incubation at 37 ℃, but not at 4 ℃ Two or 3 days' incubation with high glucose concentration (20.5 mM) increased the amounts of EBA in the lower wells significantly. Incubation with phorbol myristate acetate (PMA) also increased the amounts of EBA in the lower wells. This may suggest that high glucose levels increased albumin transcytosis through the activation of protein kinase C. This glucose-induced albumin transcytosis may explain in part, the increased vascular permeability in diabetic patients.
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
言語
言語 jpn
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346888X
収録物識別子タイプ ISSN
収録物識別子 0289-1530
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 17, 号 2, p. 89-94
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