{"_buckets": {"deposit": "b3f2fa7e-9244-4745-8ae2-54e7bdc72b65"}, "_deposit": {"id": "2016243", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2016243"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02016243", "sets": ["1642838227712", "1642838412624"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "[\u4f9d\u983c\u7dcf\u8aac]HPV infection and squamous differentiation of lung cancer cells among patients in Okinawa, Japan", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kinjo, Takao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "\u91d1\u57ce, \u8cb4\u592b", "creatorNameLang": "ja"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "ja", "subitem_1522651041219": "\u7409\u7403\u533b\u5b66\u4f1a"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "lung cancer"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Okinawa"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "squamous cell carcinoma"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "adenocarcinoma"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "HPV"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "We reviewed histopathological reports of lung cancer cases in Okinawa, Japan and found a strong association between human papillomavirus (HPV) infection and squamous differentiation of lung cancer cells. On the basis of these observations, we hypothesized that HPV infection induces squamous differentiation of adenocarcinoma cells. To prove our hypothesis and determine the molecular basis of the phenomenon, we conducted a series of in vitro and in vivo studies. Cultured adenocarcinoma cells were transfected with the entire HPV genome and the cells were injected into SCID mice to form tumors, which were used for subsequent histopathological and biochemical analyses. The HPV-transfected cells showed no tubular structure but solid proliferation with expression of squamous differentiation markers such as high molecular weight keratin and involucrin. In particular, cells expressing HPV E2, E6, and E7 genes exhibited more marked morphological changes with increased expression of squamous differentiation markers. These experiments demonstrated that HPV gene expression in adenocarcinoma cells induces squamous differentiation. Although the present studies are only preliminary analyses, our findings have identified a new and specific aspect of lung cancer in cases in Okinawa. More precise and thorough evaluations are required. Since the diseases in Okinawa are specific to mainland Japan, further investigations may lead to the discovery of new mechanisms of disease and thereby contribute to health promotion of people in Okinawa.", "subitem_description_type": "Other"}, {"subitem_description": "\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u533b\u5b66\u4f1a"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1346-888X"}, {"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0289-1530"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN10369445"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u533b\u5b66\u4f1a\u8a8c = Ryukyu Medical Journal"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "1-4", "bibliographicPageEnd": "11", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "30"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "v30(1-4)p1.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2016243/files/v30(1-4)p1.pdf"}, "version_id": "99d49ddb-51fe-4619-92a5-2dc56ac2f922"}]}, "item_title": "[\u4f9d\u983c\u7dcf\u8aac]HPV infection and squamous differentiation of lung cancer cells among patients in Okinawa, Japan", "item_type_id": "15", "owner": "1", "path": ["1642838227712", "1642838412624"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002016243", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2012-12-10"}, "publish_date": "2012-12-10", "publish_status": "0", "recid": "2016243", "relation": {}, "relation_version_is_last": true, "title": ["[\u4f9d\u983c\u7dcf\u8aac]HPV infection and squamous differentiation of lung cancer cells among patients in Okinawa, Japan"], "weko_shared_id": -1}