| Item type |
デフォルトアイテムタイプ（フル）(1) |
| 公開日 |
2019-11-29 |
| タイトル |
|
|
タイトル |
Telmisartan inhibits PAI-1 mRNA expression independently of peroxisome proliferator-activated receptor γ in vascular endothelial cells |
|
言語 |
ja |
| 作成者 |
Uehara, Ken
Sunagawa, Masanori
Nakamura-Higa, Mariko
Suzuki, Mikio
Kosugi, Tadayoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
琉球医学会 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
telmisartan |
| 主題 |
|
|
言語 |
ja |
|
主題Scheme |
Other |
|
主題 |
peroxisome proliferator-activated receptor γ |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
angiotensin II type 1 receptor antagonist |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
vascular endothelial cells |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
plasminogen activator inhibitor 1 |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
To investigate whether telmisartan (TMS), a unique angiotensin II type 1 receptor(AT1R) antagonist with selective peroxisome proliferator-activated receptor (PPAR) γ- modulating activity, inhibits plasminogen activator inhibitor 1 (PAI-1) and/or stimulates issue-type plasminogen activator (t-PA) mRNA expression in vascular endothelial cells(VECs), we cultured VECs obtained from the thoracic aorta of Long Evans Tokushima Otsuka rats by the explant method. mRNA expression was measured using comparative reverse transcription polymerase chain reaction. The binding activity of PPARγ to its coactivator (CREB-binding protein) was determined by enzyme-linked immunosorbent assay. The synthetic PPARγ agonists troglitazone (TRO) and TMS activated PPARγ with EC_50 values of 0.95 and 161μM, respectively, under cell-free conditions. PPARγ mRNA was constitutively expressed in cultured VECs, unlike PPARα and PPARβ. PAI-1 mRNA expression was significantly downregulated by TMS, and this downregulation was not abolished by the presence of the PPARγ antagonist GW9662. The expression of t-PA mRNA was not altered by TMS treatment. TRO had no effect on PAI-1 and t-PA mRNA expressions.PAI-1 mRNA expression was significantly decreased by GW9662. In conclusion, TMS inhibited PAI-1 mRNA expression independently of PPARγ activation. Intrinsic PPARγ agonists, but not extrinsic PPARγ agonists, may be involved in basal mRNA expression of PAI-1 in VECs |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
| 出版者 |
|
|
出版者 |
琉球医学会 |
|
言語 |
ja |
| 出版者 |
|
|
出版者 |
Ryukyu Medical Association |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1346-888X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10369445 |
| 収録物名 |
|
|
収録物名 |
琉球医学会誌 = Ryukyu Medical Journal |
|
言語 |
ja |
| 書誌情報 |
巻 37,
号 1-4,
p. 41-50
|
Vol37(1-4)p41.pdf
