{"_buckets": {"deposit": "082ddeea-02fc-4a8a-a810-91553c67c0e5"}, "_deposit": {"created_by": 8, "id": "2017923", "owner": "8", "owners": [8], "owners_ext": {"displayname": "ryukyu_lib", "username": null}, "pid": {"revision_id": 0, "type": "depid", "value": "2017923"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02017923", "sets": ["1642838338003", "1642838409905"]}, "author_link": [], "control_number": "2017923", "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Spontaneous pulmonary emphysema in mice lacking all three nitric oxide synthase isoforms", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kato, Kaori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tsutsui, Masato", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Noguchi, Shingo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Iha, Yukitoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Naito, Keisuke", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ogoshi, Takaaki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nishida, Chinatsu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tahara, Masahiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yamashita, Hirotaka", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Wang, Ke\u2011Yong", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Toyohira, Yumiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yanagihara, Nobuyuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Masuzaki, Hiroaki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shimokawa, Hiroaki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Akihide, Tanimoto", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yatera, Kazuhiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "en", "subitem_1522650727486": "https://creativecommons.org/licenses/by/4.0/", "subitem_1522651041219": "Creative Commons Attribution 4.0 International  License"}, {"subitem_1522650717957": "en", "subitem_1522651041219": "\u00a9 The Author(s) 2021"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "The roles of endogenous nitric oxide (NO) derived from the entire NO synthases (NOSs) system have yet to be fully elucidated. We addressed this issue in mice in which all three NOS isoforms were deleted. Under basal conditions, the triple n/i/eNOSs\u2212/\u2212 mice displayed significantly longer mean alveolar linear intercept length, increased alveolar destructive index, reduced lung elastic fiber content, lower lung field computed tomographic value, and greater end-expiratory lung volume as compared with wild-type (WT) mice. None of single NOS\u2212/\u2212 or double NOSs\u2212/\u2212 genotypes showed such features. These findings were observed in the triple n/i/eNOSs\u2212/\u2212 mice as early as 4 weeks after birth. Cyclopaedic and quantitative comparisons of mRNA expression levels between the lungs of WT and triple n/i/eNOSs\u2212/\u2212 mice by cap analysis of gene expression (CAGE) revealed that mRNA expression levels of three Wnt ligands and ten Wnt/\u03b2-catenin signaling components were signifcantly reduced in the lungs of triple n/i/eNOSs\u2212/\u2212 mice. These results provide the first direct evidence that complete disruption of all three NOS genes results in spontaneous pulmonary emphysema in juvenile mice in vivo possibly through down-regulation of the Wnt/\u03b2-catenin signaling pathway, demonstrating a novel preventive role of the endogenous NO/NOS system in the occurrence of pulmonary emphysema.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "en", "subitem_1522300316516": "Springer Nature"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "EISSN", "subitem_1522646572813": "2045-2322"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "Scientific Reports"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-11-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "22088", "bibliographicVolumeNumber": "11"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1038/s41598-021-01453-6"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2022-05-09"}], "download_preview_message": "", "file_order": 0, "filename": "s41598-021-01453-6.pdf", "filesize": [{"value": "2.4 MB"}], "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 2400000.0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2017923/files/s41598-021-01453-6.pdf"}, "version_id": "3086e535-af57-4ab8-8c54-fa3908c91f5e"}]}, "item_title": "Spontaneous pulmonary emphysema in mice lacking all three nitric oxide synthase isoforms", "item_type_id": "59", "owner": "8", "path": ["1642838338003", "1642838409905"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002017923", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-05-12"}, "publish_date": "2022-05-12", "publish_status": "0", "recid": "2017923", "relation": {}, "relation_version_is_last": true, "title": ["Spontaneous pulmonary emphysema in mice lacking all three nitric oxide synthase isoforms"], "weko_shared_id": -1}