Item type |
琉球大学リポジトリ登録用アイテムタイプ(1) |
公開日 |
2022-05-12 |
タイトル |
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タイトル |
Spontaneous pulmonary emphysema in mice lacking all three nitric oxide synthase isoforms |
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言語 |
en |
作成者 |
Kato, Kaori
Tsutsui, Masato
Noguchi, Shingo
Iha, Yukitoshi
Naito, Keisuke
Ogoshi, Takaaki
Nishida, Chinatsu
Tahara, Masahiro
Yamashita, Hirotaka
Wang, Ke‑Yong
Toyohira, Yumiko
Yanagihara, Nobuyuki
Masuzaki, Hiroaki
Shimokawa, Hiroaki
Akihide, Tanimoto
Yatera, Kazuhiro
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
en |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
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権利情報 |
Creative Commons Attribution 4.0 International License |
権利情報 |
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言語 |
en |
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権利情報 |
© The Author(s) 2021 |
内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
The roles of endogenous nitric oxide (NO) derived from the entire NO synthases (NOSs) system have yet to be fully elucidated. We addressed this issue in mice in which all three NOS isoforms were deleted. Under basal conditions, the triple n/i/eNOSs−/− mice displayed significantly longer mean alveolar linear intercept length, increased alveolar destructive index, reduced lung elastic fiber content, lower lung field computed tomographic value, and greater end-expiratory lung volume as compared with wild-type (WT) mice. None of single NOS−/− or double NOSs−/− genotypes showed such features. These findings were observed in the triple n/i/eNOSs−/− mice as early as 4 weeks after birth. Cyclopaedic and quantitative comparisons of mRNA expression levels between the lungs of WT and triple n/i/eNOSs−/− mice by cap analysis of gene expression (CAGE) revealed that mRNA expression levels of three Wnt ligands and ten Wnt/β-catenin signaling components were signifcantly reduced in the lungs of triple n/i/eNOSs−/− mice. These results provide the first direct evidence that complete disruption of all three NOS genes results in spontaneous pulmonary emphysema in juvenile mice in vivo possibly through down-regulation of the Wnt/β-catenin signaling pathway, demonstrating a novel preventive role of the endogenous NO/NOS system in the occurrence of pulmonary emphysema. |
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言語 |
en |
出版者 |
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言語 |
en |
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出版者 |
Springer Nature |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-021-01453-6 |
収録物識別子 |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2045-2322 |
収録物名 |
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言語 |
en |
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収録物名 |
Scientific Reports |
書誌情報 |
巻 11,
p. 22088,
発行日 2021-11-11
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