{"_buckets": {"deposit": "242d79f6-bb28-436c-a219-e327feed4f25"}, "_deposit": {"created_by": 8, "id": "2019640", "owner": "8", "owners": [8], "owners_ext": {"displayname": "ryukyu_lib", "username": null}, "pid": {"revision_id": 0, "type": "depid", "value": "2019640"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02019640", "sets": ["1642838338003", "1642838407795"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ebi, Nana", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Miyagi-Shiohira, Chika", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hamada, Eri", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tamaki, Yoshihito", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Masamoto, Mariko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Makishi, Erika", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nakashima, Yoshiki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Naoya", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Saitoh, Issei", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Watanabe, Masami", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Noguchi, Yasufumi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kinjo, Takao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Noguchi, Hirofumi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "en", "subitem_1522651041219": "© The Author(s) 2018"}, {"subitem_1522650717957": "en", "subitem_1522651041219": "Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage)."}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "islet transplantation"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "islet isolation"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "islet purification"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "bottle purification"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "continuous density gradient"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from porcine pancreas. In this study, we evaluated the methods for making a continuous density gradient and loading tissue. One method involved loading digested tissue on top of a continuous density gradient (top loading). The other method involved mixing digested tissue with low-density solution and then making a continuous gradient (mixed loading). There were no significant differences between the 2 purification methods in terms of the islet yield, rate of viability or purity, score, or in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these 2 methods of islet purification.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "en", "subitem_1522300316516": "SAGE Publishing"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "EISSN", "subitem_1522646572813": "2155-1790"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "Cell Medicine"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-05", "bibliographicIssueDateType": "Issued"}, "bibliographicVolumeNumber": "10"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1177/2155179017733090"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2023-03-10"}], "download_preview_message": "", "file_order": 0, "filename": "2155179017733090.pdf", "filesize": [{"value": "284 KB"}], "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 284000.0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2019640/files/2155179017733090.pdf"}, "version_id": "7dd11d7e-196c-41c2-8064-6e85c115882b"}]}, "item_title": "Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue", "item_type_id": "59", "owner": "8", "path": ["1642838338003", "1642838407795"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002019640", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2023-03-10"}, "publish_date": "2023-03-10", "publish_status": "0", "recid": "2019640", "relation": {}, "relation_version_is_last": true, "title": ["Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue"], "weko_shared_id": -1}