Item type |
琉球大学リポジトリ登録用アイテムタイプ(1) |
公開日 |
2023-03-10 |
タイトル |
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タイトル |
Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue |
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言語 |
en |
作成者 |
Ebi, Nana
Miyagi-Shiohira, Chika
Hamada, Eri
Tamaki, Yoshihito
Masamoto, Mariko
Makishi, Erika
Nakashima, Yoshiki
Kobayashi, Naoya
Saitoh, Issei
Watanabe, Masami
Noguchi, Yasufumi
Kinjo, Takao
Noguchi, Hirofumi
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
en |
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権利情報 |
© The Author(s) 2018 |
権利情報 |
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言語 |
en |
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権利情報 |
Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
islet transplantation |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
islet isolation |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
islet purification |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
bottle purification |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
continuous density gradient |
内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from porcine pancreas. In this study, we evaluated the methods for making a continuous density gradient and loading tissue. One method involved loading digested tissue on top of a continuous density gradient (top loading). The other method involved mixing digested tissue with low-density solution and then making a continuous gradient (mixed loading). There were no significant differences between the 2 purification methods in terms of the islet yield, rate of viability or purity, score, or in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these 2 methods of islet purification. |
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言語 |
en |
出版者 |
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言語 |
en |
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出版者 |
SAGE Publishing |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1177/2155179017733090 |
収録物識別子 |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2155-1790 |
収録物名 |
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言語 |
en |
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収録物名 |
Cell Medicine |
書誌情報 |
巻 10,
発行日 2018-05
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