| Item type |
琉球大学リポジトリ登録用アイテムタイプ(1) |
| 公開日 |
2023-03-29 |
| タイトル |
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|
タイトル |
Analysis of human clinical and environmental Leptospira to elucidate the eco-epidemiology of leptospirosis in Yaeyama, subtropical Japan |
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言語 |
en |
| 作成者 |
Sato, Yukuto
Hermawan, Idam
Kakita, Tetsuya
Okano, Sho
Imai, Hideyuki
Nagai, Hiroto
Kimura, Ryosuke
Yamashiro, Tetsu
Kajita, Tadashi
Toma, Claudia
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
言語 |
en |
|
権利情報 |
© 2022 Sato et al. |
| 権利情報 |
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言語 |
en |
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権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 内容記述 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Background
Leptospirosis, a zoonosis caused by species in the spirochete genus Leptospira, is endemic to the Yaeyama region in Okinawa, subtropical Japan. Species of the P1 subclade “virulent” group, within the genus Leptospira, are the main etiological agents of leptospirosis in Okinawa. However, their environmental persistence is poorly understood. This study used a combination of bacterial isolation and environmental DNA (eDNA) metabarcoding methods to understand the eco-epidemiology of leptospirosis in this endemic region.
Findings
Polymerase chain reaction (PCR) characterized twelve human clinical L. interrogans isolates belonging to the P1 subclade “virulent” subgroup and 11 environmental soil isolates of the P1subclade “low virulent” subgroup (genetically related to L. kmetyi, n = 1; L. alstonii, n = 4; L. barantonii, n = 6) from the Yaeyama region targeting four virulence-related genes (lipL32, ligA, ligB and lpxD1). Clinical isolates were PCR positive for at least three targeted genes, while all environmental isolates were positive only for lipL32. Analysis of infected renal epithelial cells with selected clinical and environmental strains, revealed the disassembly of cell-cell junctions for the Hebdomadis clinical strain serogroup. Comparison of leptospiral eDNA during winter and summer identified operational taxonomic units corresponding to the species isolated from soil samples (L. kmetyi and L. barantonii) and additional P2 subclade species (L. licerasiae, L. wolffii-related, among others) that were not detected by soil cultivation. Total Leptospira read counts were higher in summer than in winter and the analysis of leptospiral/animal eDNA relationship suggested Rattus spp. as a potential reservoir animal.
Conclusion
Our study demonstrated high environmental Leptospira diversity in the Yaeyama region, particularly during summer, when most of the leptospirosis cases are reported. In addition, several Leptospira species with pathogenic potential were identified that have not yet been reported in Yaeyama; however, the environmental persistence of P1 subclade species previously isolated from human clinical cases in this region was absent, suggesting the need of further methodology development and surveillance. |
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言語 |
en |
| 出版者 |
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出版者 |
Public Library of Science |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pntd.0010234 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1935-2727 |
| 収録物識別子 |
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収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1935-2735 |
| 収録物名 |
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|
収録物名 |
PLoS Neglected Tropical Diseases |
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言語 |
en |
| 書誌情報 |
巻 16,
号 3,
発行日 2022-03
|
journal.pntd.0010234.pdf (3.2 MB)
