{"_buckets": {"deposit": "7be053ab-c621-464b-8697-d5868b0c15f3"}, "_deposit": {"created_by": 8, "id": "2019665", "owner": "8", "owners": [8], "owners_ext": {"displayname": "ryukyu_lib", "username": null}, "pid": {"revision_id": 0, "type": "depid", "value": "2019665"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02019665", "sets": ["1642838409905", "1642838338003"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sebastián, Isabel", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Okura, Nobuhiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Humbel, M. Bruno", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Xu, Jun", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hermawan, Idam", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Matsuura, Chiaki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hall, Malgorzata", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Takayama, Chitoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yamashiro, Tetsu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nakamura, Shuichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Toma, Claudia", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "en", "subitem_1522651041219": "© 2021 The Authors"}, {"subitem_1522650717957": "en", "subitem_1522650727486": "https://creativecommons.org/licenses/by-nc/4.0/", "subitem_1522651041219": "This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes."}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "apical junctional complex"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Leptospira interrogans"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "renal epithelial cells"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Bacterial pathogens have evolved multiple strategies to disassemble epithelial cell apical junctional complexes (AJCs) and infect epithelial cells. Leptospirosis is a widespread zoonotic infection, mainly caused by Leptospira interrogans, and its dissemination across host cell barriers is essential for its pathogenesis. However, the mechanism of bacterial dissemination across epithelial cell barriers remains poorly characterised. In this study, we analysed the interaction of L. interrogans with renal proximal tubule epithelial cells (RPTECs) and found that at 24 hr post-infection, L. interrogans remain in close contact with the plasma membrane of the RPTEC by extracellularly adhering or crawling. Leptospira interrogans cleaved E-cadherin and induced its endocytosis with release of the soluble N-terminal fragment into the extracellular medium. Concomitantly, a gradual decrease in transepithelial electrical resistance (TEER), mislocalisation of AJC proteins (occludin, claudin-10, ZO-1, and cingulin) and cytoskeletal rearrangement were observed. Inhibition of clathrin-mediated E-cadherin endocytosis prevented the decrease in TEER. We showed that disassembly of AJCs in epithelial cells and transmigration of bacteria through the paracellular route are important for the dissemination of L. interrogans in the host.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "en", "subitem_1522300316516": "John Wiley \u0026 Sons Ltd."}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "PISSN", "subitem_1522646572813": "1462-5814"}, {"subitem_1522646500366": "EISSN", "subitem_1522646572813": "1462-5822"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "Cellular Microbiology"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicVolumeNumber": "23"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1111/cmi.13343"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2023-03-29"}], "download_preview_message": "", "file_order": 0, "filename": "Cellular Microbiology - 2021 - Sebasti n - Disassembly of the apical junctional complex during the transmigration of.pdf", "filesize": [{"value": "13.3 MB"}], "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 13300000.0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2019665/files/Cellular Microbiology - 2021 - Sebasti n - Disassembly of the apical junctional complex during the transmigration of.pdf"}, "version_id": "8709bca8-14bb-4558-8bd3-f0c297600886"}]}, "item_title": "Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells", "item_type_id": "59", "owner": "8", "path": ["1642838338003", "1642838409905"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002019665", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2023-03-29"}, "publish_date": "2023-03-29", "publish_status": "0", "recid": "2019665", "relation": {}, "relation_version_is_last": true, "title": ["Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells"], "weko_shared_id": -1}