| Item type |
琉球大学リポジトリ登録用アイテムタイプ(1) |
| 公開日 |
2023-03-29 |
| タイトル |
|
|
タイトル |
Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells |
|
言語 |
en |
| 作成者 |
Sebastián, Isabel
Okura, Nobuhiko
Humbel, M. Bruno
Xu, Jun
Hermawan, Idam
Matsuura, Chiaki
Hall, Malgorzata
Takayama, Chitoshi
Yamashiro, Tetsu
Nakamura, Shuichi
Toma, Claudia
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2021 The Authors |
| 権利情報 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by-nc/4.0/ |
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
apical junctional complex |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Leptospira interrogans |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
renal epithelial cells |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Bacterial pathogens have evolved multiple strategies to disassemble epithelial cell apical junctional complexes (AJCs) and infect epithelial cells. Leptospirosis is a widespread zoonotic infection, mainly caused by Leptospira interrogans, and its dissemination across host cell barriers is essential for its pathogenesis. However, the mechanism of bacterial dissemination across epithelial cell barriers remains poorly characterised. In this study, we analysed the interaction of L. interrogans with renal proximal tubule epithelial cells (RPTECs) and found that at 24 hr post-infection, L. interrogans remain in close contact with the plasma membrane of the RPTEC by extracellularly adhering or crawling. Leptospira interrogans cleaved E-cadherin and induced its endocytosis with release of the soluble N-terminal fragment into the extracellular medium. Concomitantly, a gradual decrease in transepithelial electrical resistance (TEER), mislocalisation of AJC proteins (occludin, claudin-10, ZO-1, and cingulin) and cytoskeletal rearrangement were observed. Inhibition of clathrin-mediated E-cadherin endocytosis prevented the decrease in TEER. We showed that disassembly of AJCs in epithelial cells and transmigration of bacteria through the paracellular route are important for the dissemination of L. interrogans in the host. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
John Wiley & Sons Ltd. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1111/cmi.13343 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1462-5814 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1462-5822 |
| 収録物名 |
|
|
収録物名 |
Cellular Microbiology |
|
言語 |
en |
| 書誌情報 |
巻 23,
号 9,
発行日 2021-09
|
Cellular Microbiology - 2021 - Sebasti n - Disassembly of the apical junctional complex during the transmigration of.pdf (13.3 MB)
