| Item type |
琉球大学リポジトリ登録用アイテムタイプ(1) |
| 公開日 |
2024-10-17 |
| タイトル |
|
|
タイトル |
Addition of l-carnitine to the freezing extender improves post-thaw sperm quality of Okinawan native Agu pig |
|
言語 |
en |
| 作成者 |
Tatemoto, Hideki
| en |
Tatemoto, Hideki
Faculty of Agriculture, University of the Ryukyus
|
| ja |
琉球大学農学部
|
Search repository
Osokoshi, Naoto
| en |
Osokoshi, Naoto
Faculty of Agriculture, University of the Ryukyus
|
| ja |
琉球大学農学部
|
Search repository
Hirai, Moe
| en |
Hirai, Moe
Faculty of Agriculture, University of the Ryukyus
|
| ja |
琉球大学農学部
|
Search repository
Masuda, Yusuke
| en |
Masuda, Yusuke
Faculty of Agriculture, University of the Ryukyus
|
| ja |
琉球大学農学部
|
Search repository
Konno, Toshihiro
| en |
Konno, Toshihiro
Faculty of Agriculture, University of the Ryukyus
|
| ja |
琉球大学農学部
|
Search repository
Yamanaka, Kenichi
| en |
Yamanaka, Kenichi
Faculty of Agriculture, Saga University
|
| ja |
佐賀大学農学部
|
Search repository
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja |
|
権利情報 |
This Is an Open Access Article Under the CC BY-NC-ND License. |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Boar spermatozoa |
| 主題 |
|
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
L-carnitine |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cryoinjury |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cell damage |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cryopreservation |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Okinawan native Agu pig |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The objective of the present study was to establish whether the addition of l-carnitine (LC), which exhibits antioxidant activity, to the freezing extender improves the quality of cryopreserved Okinawan native Agu pig sperm. Ejaculated sperm frozen in an extender supplemented with 0, 1, 2.5, or 5 mM LC was thawed, and the integrities of mitochondria and the plasmalemma and other sperm characteristics were evaluated. The treatment with different concentrations of LC effectively improved sperm motility, mitochondrial and plasmalemmal integrities, and the proteolytic activity of acrosomal contents after freeze-thawing (P < 0.05). The proportion of post-thaw sperm possessing intact mitochondria and plasmalemma and higher proteolytic activity of acrosomal contents was markedly higher among sperm frozen in the presence of 2.5 mM LC than among sperm frozen in the extender without LC (P < 0.05). Furthermore, although the addition of LC to the freezing extender had no effect on disturbance of DNA damage and caspase activity, sperm treated with 2.5 mM LC during freezing exhibited significantly higher penetrability into matured oocytes in vitro than untreated sperm. Collectively, these results indicate that the addition of LC to the freezing extender effectively improved the post-thaw quality of Agu pig sperm by preventing mitochondrial dysfunction caused by oxidative stress during cryopreservation. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 識別子 |
|
|
識別子 |
https://doi.org/10.1016/j.theriogenology.2021.12.030 |
|
識別子タイプ |
DOI |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0093-691X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1879-3231 |
| 収録物名 |
|
|
収録物名 |
Theriogenology |
|
言語 |
en |
| 書誌情報 |
巻 188,
p. 170-176,
発行日 2022-08
|
T_No188p170.pdf (628 KB)
