| Item type |
琉球大学リポジトリ登録用アイテムタイプ(1) |
| 公開日 |
2024-11-22 |
| タイトル |
|
|
タイトル |
Live imaging of center of calcification formation during septum development in primary polyps of Acropora digitifera |
|
言語 |
en |
| 作成者 |
Ohno, Yoshikazu
| en |
Ohno, Yoshikazu
School of Marine Biosciences, Kitasato University
|
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Takahashi, Anan
| en |
Takahashi, Anan
School of Marine Biosciences, Kitasato University
|
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Tsutsumi, Motosuke
Kubota, Azusa
| en |
Kubota, Azusa
School of Marine Biosciences, Kitasato University
JEOL Ltd.
|
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Iguchi, Akira
| en |
Iguchi, Akira
National Institute of Advanced Industrial Science and Technology
|
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Iijima, Mariko
| en |
Iijima, Mariko
National Institute of Advanced Industrial Science and Technology
|
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Mizusawa, Nanami
| en |
Mizusawa, Nanami
School of Marine Biosciences, Kitasato University
|
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Nakamura, Takashi
| en |
Nakamura, Takashi
Graduate School of Engineering and Science, University of the Ryukyus
Tropical Biosphere Research Center, University of the Ryukyus
|
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Suzuki, Atsushi
| en |
Suzuki, Atsushi
National Institute of Advanced Industrial Science and Technology
|
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Suzuki, Michio
| en |
Suzuki, Michio
Graduate School of Agricultural and Life Sciences, The University of Tokyo
|
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Yasumoto, Jun
Watabe, Shugo
Sakai, Kazuhiko
| en |
Sakai, Kazuhiko
Tropical Biosphere Research Center, University of the Ryukyus
|
Search repository
Nemoto, Tomomi
Yasumoto, Ko
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2024 Ohno, Takahashi, Tsutsumi, Kubota, Iguchi, Iijima, Mizusawa, Nakamura, Suzuki, Suzuki, Yasumoto, Watabe, Sakai, Nemoto and Yasumoto. |
| 権利情報 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
coral |
| 主題 |
|
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
calcification |
| 主題 |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
septa |
| 主題 |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
centers of calcification (CoC) |
| 主題 |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
two-photon microscopy (2-PM) |
| 主題 |
|
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
calcein |
| 内容記述 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Recent studies have revealed that stony corals create their extracellular skeletons via biologically controlled calcification, in which amorphous calcium carbonate (ACC), regarded as precursors of aragonite crystals, have been observed at nanoscale using electron microscopy. However, the exact mechanism by which ACC is generated, and how it contributes to skeletal growth in coral calcifying tissue, remains enigmatic. The septal skeleton of an individual polyp is composed of radially aligned plates extending upward from the aboral calcifying tissue. This structure includes microstructure known as the centers of calcification (CoC). However, despite its importance, direct in vivo observation of septal growth has not been reported. Observations under transmitted illumination using polarized light microscopy on calcifying tissue of young Acropora digitifera revealed small crystals, a few micrometers in size, that accompany subtle movements and that emerge exclusively on the inner wall of the pocket in extracellular calcifying fluid (ECF). Crystal growth initiated from small, scattered crystals on a glass plate resembles this phenomenon observed in coral skeletons. Time-lapse photographs of 12 individuals in early primary polyp settlement revealed this process in three individuals, documenting 13 of these crystal events. This phenomenon occurred solely at the bases of subsequently formed septa. These crystals differ notably from fusiform crystals and from dumbbell-like or rod-like crystals growing individually. Upright two-photon microscopy captured movement of sub-micron-sized fluorescent calcein-accumulating particles, emphasizing their presence on the surface of the growing fronts of septa. Methodological advances that facilitate comprehensive in vivo observation of sub-micron-sized structures, calcein-accumulating particles to the skeleton, are needed to develop a more detailed understanding of coral skeletal growth. |
|
言語 |
en |
| 出版者 |
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出版者 |
Frontiers Media |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3389/fmars.2024.1406446 |
| 収録物識別子 |
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収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2296-7745 |
| 収録物名 |
|
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収録物名 |
Frontiers in Marine Science |
|
言語 |
en |
| 書誌情報 |
巻 11,
p. 1-13,
発行日 2024-08-06
|
O_No11p1_2024.pdf (5 MB)
