Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2007-12-10 |
タイトル |
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タイトル |
Mechanisms of Legionella pneumophila-induced interleukin-8 expression in human lung epithelial cells |
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言語 |
en |
作成者 |
Teruya, Hiromitsu
Higa, Futoshi
Akamine, Morikazu
Ishikawa, Chie
Okudaira, Taeko
Tomimori, Koh
Mukaida, Naofumi
Tateyama, Masao
Heuner, Klaus
Fujita, Jiro
Mori, Naoki
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Background: Legionella pneumophila is a facultative intracellular bacterium, capable of replicating within the phagosomes of macrophages and monocytes, but little is known about its interaction with human lung epithelial cells. We investigated the effect of L. pneumophila on the expression of interleukin-8 (IL-8) in human A549 alveolar and NCI-H292 tracheal epithelial cell lines. \nResults: Infection of L. pneumophila strain, but not heat-killed strain, resulted in upregulation of IL-8. IL-8 mRNA expression was induced immediately after the infection and its signal became gradually stronger until 24 h after infection. On the other hand, IL-8 expression in A549 cells infected with L. pneumophila lacking a functional type IV secretion system was transient. The IL-8 expression was slightly induced at 16 h and increased at 24 h after infection with flagellin-deficient Legionella. Activation of the IL-8 promoter by L. pneumophila infection occurred through the action of nuclear factor-κB (NF-κB). Transfection of dominant negative mutants of NF-κB-inducing kinase, IκB kinase and IκB inhibited L. pneumophila-mediated activation of IL-8 promoter. Treatment with hsp90 inhibitor suppressed L. pneumophila-induced IL-8 mRNA due to deactivation of NF-κB. \nConclusions: Collectively, these results suggest that L. pneumophila induces activation of NF-κB through an intracellular signaling pathway that involves NF-κB-inducing kinase and IκB kinase, leading to IL-8 gene transcription, and that hsp90 acts as a crucial regulator in L. pneumophila-induced IL-8 expression, presumably contributing to immune response in L. pneumophila. The presence of flagellin and a type IV secretion system are critical for Legionella to induce IL-8 expression in lung epithelial cells. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
en |
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出版者 |
BioMed Central |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
識別子 |
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識別子 |
http://hdl.handle.net/20.500.12000/2607 |
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識別子タイプ |
HDL |
関連情報 |
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識別子タイプ |
URI |
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関連識別子 |
http://www.biomedcentral.com/1471-2180/7/102/ |
関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1186/1471-2180-7-102 |
関連情報 |
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識別子タイプ |
PMID |
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関連識別子 |
18034886 |
収録物識別子 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12035256 |
収録物名 |
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言語 |
en |
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収録物名 |
BMC microbiology |
書誌情報 |
巻 7,
号 1,
p. 102
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