{"_buckets": {"deposit": "47bd4cb2-ffee-4b92-8380-8c8a0d5b319e"}, "_deposit": {"id": "2003114", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003114"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003114", "sets": ["1642837912269", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "ハブ毒のコラゲナーゼに関する研究(農芸化学科)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on collagenase of Habu (Trimeresurus flavoviridis) venom (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "知念, 功", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "石松, 亮", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "福田, 亘博", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ishimatsu, Akira", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Fukuda, Nobuhiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "ハブ毒を不溶性コラーゲンに作用し, 生成してくる可溶性ペプタイド中のヒドロキシプロリン含量を定量することにより, ハブ毒コラゲナーゼ活性を求めた。この酵素の至適pHは, 8.0であり, 至適温度は, 60℃であった。この不溶性コラーゲンにこの酵素を吸着させ(30℃, 60分反応), 遠心し, 1M食塩溶液で溶出し, 本酵素を精製した。本酵素の吸着率は, 80%であった。また溶出率も80%であった。このコラーゲンと粗毒の添加割合は, 300 : 1の時本酵素の吸着率は, 最大であった。この精製酵素をさらにセファデックスG-75,G-150ゲルカラムクロマトグラフィーにかけ純化した結果, その純化倍率は, 約1.13倍になった。この純化酵素は, ディスクポリアクリルアミドゲル電気泳動的に2個以上のバンドが見られ, またコラゲナーゼ活性以外にカゼイン水解活性も見られた。", "subitem_description_type": "Other"}, {"subitem_description": "Collagenase activity of Habu venom was measured the amount of hydroxyproline of soluble peptide liberated by reaction of the venom and insoluble collagen (60℃, 30 min). The enzyme activity was maximum at pH 8.0 and at 60℃. The purification of the enzyme was carried out by being adsorbed onto insoluble collagen (30℃, 60min), centrifuged, and eluted with 1M sodium cloride solution. Rate of adsorption of the enzyme was maximum, 80% for total activity, when ratio of insoluble collagen to the enzyme was 300 : 1,and the elution also 80% for activity of the enzyme adsorbed. Further purification of the enzyme fraction by Sephadex G-75 and G-150 column chromatography was found to be a 6.5% over all yield and a 1.13 fold increase in specific activity. The purified enzyme revealed two more bands by disc gel electrophoresis and hydrolysed casein.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4085"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1980-11-29", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "27", "bibliographicPageEnd": "56", "bibliographicPageStart": "43"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162043.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003114/files/KJ00000162043.pdf"}, "version_id": "a5e8873d-fdef-47a3-b993-3a90705537d0"}]}, "item_title": "ハブ毒のコラゲナーゼに関する研究(農芸化学科)", "item_type_id": "15", "owner": "1", "path": ["1642837912269", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4085", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003114", "relation": {}, "relation_version_is_last": true, "title": ["ハブ毒のコラゲナーゼに関する研究(農芸化学科)"], "weko_shared_id": -1}