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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 27号
  1. 部局別インデックス
  2. 農学部

ハブ毒のコラゲナーゼに関する研究(農芸化学科)

http://hdl.handle.net/20.500.12000/4085
http://hdl.handle.net/20.500.12000/4085
f065474e-5cb5-4aab-a48c-bfb38d966368
名前 / ファイル ライセンス アクション
KJ00000162043.pdf KJ00000162043.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル ハブ毒のコラゲナーゼに関する研究(農芸化学科)
言語 ja
作成者 知念, 功

× 知念, 功

ja 知念, 功

石松, 亮

× 石松, 亮

ja 石松, 亮

福田, 亘博

× 福田, 亘博

ja 福田, 亘博

Chinen, Isao

× Chinen, Isao

en Chinen, Isao

Ishimatsu, Akira

× Ishimatsu, Akira

en Ishimatsu, Akira

Fukuda, Nobuhiro

× Fukuda, Nobuhiro

en Fukuda, Nobuhiro

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 ハブ毒を不溶性コラーゲンに作用し, 生成してくる可溶性ペプタイド中のヒドロキシプロリン含量を定量することにより, ハブ毒コラゲナーゼ活性を求めた。この酵素の至適pHは, 8.0であり, 至適温度は, 60℃であった。この不溶性コラーゲンにこの酵素を吸着させ(30℃, 60分反応), 遠心し, 1M食塩溶液で溶出し, 本酵素を精製した。本酵素の吸着率は, 80%であった。また溶出率も80%であった。このコラーゲンと粗毒の添加割合は, 300 : 1の時本酵素の吸着率は, 最大であった。この精製酵素をさらにセファデックスG-75,G-150ゲルカラムクロマトグラフィーにかけ純化した結果, その純化倍率は, 約1.13倍になった。この純化酵素は, ディスクポリアクリルアミドゲル電気泳動的に2個以上のバンドが見られ, またコラゲナーゼ活性以外にカゼイン水解活性も見られた。
内容記述タイプ Other
内容記述 Collagenase activity of Habu venom was measured the amount of hydroxyproline of soluble peptide liberated by reaction of the venom and insoluble collagen (60℃, 30 min). The enzyme activity was maximum at pH 8.0 and at 60℃. The purification of the enzyme was carried out by being adsorbed onto insoluble collagen (30℃, 60min), centrifuged, and eluted with 1M sodium cloride solution. Rate of adsorption of the enzyme was maximum, 80% for total activity, when ratio of insoluble collagen to the enzyme was 300 : 1,and the elution also 80% for activity of the enzyme adsorbed. Further purification of the enzyme fraction by Sephadex G-75 and G-150 column chromatography was found to be a 6.5% over all yield and a 1.13 fold increase in specific activity. The purified enzyme revealed two more bands by disc gel electrophoresis and hydrolysed casein.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4085
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 27, p. 43-56
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