{"_buckets": {"deposit": "22222c36-a0d5-4232-aaf5-d1bfae1a53c3"}, "_deposit": {"id": "2003118", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003118"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003118", "sets": ["1642837912269", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u6ce1\u76db\u9eb9\u83cc\u306e L-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "L-Alanine : \u03b1-ketoglutarate transaminase from Aspergillus awamori; purification and properties (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u77f3\u539f, \u660c\u4fe1", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u4e0e\u90a3\u8987, \u548c\u96c4", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5f53\u5c71, \u6e05\u5584", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Ishihara, Masanobu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yonaha, Kazuo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Toyama, Seizen", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u5404\u7a2e\u30ab\u30d3\u306b\u304a\u3051\u308bL-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u6d3b\u6027\u3092\u8abf\u3079\u305f\u7d50\u679c, \u6ce1\u76db\u9eb9\u83cc(Asp. awamori IFO4122)\u3092\u8517\u7cd6-\u30da\u30d7\u30c8\u30f3\u542b\u6709\u57f9\u5730\u3067\u632f\u3068\u3046\u57f9\u990a\u3057, \u5f97\u3089\u308c\u305f\u83cc\u4f53\u306e\u7121\u7d30\u80de\u62bd\u51fa\u6db2\u4e2d\u306b\u9ad8\u3044\u9175\u7d20\u6d3b\u6027\u304c\u5f97\u3089\u308c\u305f\u3002\u672c\u9175\u7d20\u306fL-\u30a2\u30e9\u30cb\u30f3\u3068\u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u304b\u3089L-\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u3068\u30d4\u30eb\u30d3\u30f3\u9178\u3092\u751f\u6210\u3059\u308b\u30a2\u30df\u30ce\u57fa\u8ee2\u79fb\u53cd\u5fdc\u3092\u89e6\u5a92\u3057, \u672c\u53cd\u5fdc\u306e\u9006\u53cd\u5fdc\u3082\u89e6\u5a92\u3057\u305f\u3002\u672c\u9175\u7d20\u306f, \u4f9b\u8a66\u6ce1\u76db\u9eb9\u83cc\u682a\u306e\u7121\u7d30\u80de\u62bd\u51fa\u6db2\u304b\u3089\u786b\u5b89\u5206\u753b\u53ca\u3073\u5404\u7a2e\u306e\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306b\u3088\u308a55\u500d\u306b\u7cbe\u88fd\u3055\u308c\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306e\u30b2\u30eb\u6eec\u904e\u6cd5\u3067\u6c42\u3081\u305f\u5206\u5b50\u91cf\u306f104,000\u3067\u3042\u308a, \u5438\u53ce\u30b9\u30da\u30af\u30c8\u30eb\u306f280nm, 320nm\u53ca\u3073418nm\u306b\u5438\u53ce\u6975\u5927\u304c\u3042\u3063\u305f, \u7cbe\u88fd\u9175\u7d20\u306fL-\u30a2\u30e9\u30cb\u30f3\u306b\u7279\u7570\u7684\u3067, L-\u30a2\u30e9\u30cb\u30f3\u53ca\u3073\u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u306b\u5bfe\u3059\u308bkm\u5024\u306f2.9mM\u3067\u3042\u3063\u305f\u3002\u9175\u7d20\u53cd\u5fdc\u306e\u81f3\u9069pH\u306f7.0\u0026acd;8.0\u3067, \u81f3\u9069\u6e29\u5ea6\u306f45\u2103\u3067\u3042\u3063\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "The activity of L-alanine : \u03b1-ketoglutarate transaminase in the cell-free extracts of various molds has been determined. A high activity of the transaminase was found in the cell-free extract of Aspergillus awamori IFO 4122 grown on sucrose-peptone medium with shaking, and the strain was chosen for characterization and purification of the enzyme. The enzyme catalyzed reversibly the transamination between L-alanine and \u03b1-ketoglutarate producing L-glutamate and pyruvate. The enzyme was purified 55 fold from the cell-free extract, using ammonium sulfate fractionation and various chromatographic techniques. A molecular weight of 104,000 was obtained by gel filtration. The purified enzyme exhibits absorption maxima at 280nm, 320nm and 418nm, at pH 8.0. The purified enzyme was highly specific for L-alanine and the Km value for L-alanine and \u03b1-ketoglutarate was estimated to be 2.9mM. The optimal pH and temperature for the transaminase reaction were 7.0-8.0 and 45\u2103, respectively.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4089"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "27", "bibliographicPageEnd": "102", "bibliographicPageStart": "89"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162047.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003118/files/KJ00000162047.pdf"}, "version_id": "23fadff4-4ada-4bd9-94ea-18b9fb1f4fe5"}]}, "item_title": "\u6ce1\u76db\u9eb9\u83cc\u306e L-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837912269", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4089", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003118", "relation": {}, "relation_version_is_last": true, "title": ["\u6ce1\u76db\u9eb9\u83cc\u306e L-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}