{"_buckets": {"deposit": "1359c406-c392-4786-a335-d0a6f0bdebe8"}, "_deposit": {"id": "2003209", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003209"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003209", "sets": ["1642837910985", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u30aa\u30cb\u30d2\u30c8\u30c7\u80c3\u90e8\u306e\u30b3\u30e9\u30fc\u30b2\u30ca\u30fc\u30bc, \u9178\u6027\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on collagenase and acid protease in stomach of Acanthaster planci (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u56db\u65b9, \u6cbb\u4e94\u90ce", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u6c5f\u5ddd, \u7fa9\u548c", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5bae\u826f, \u751f\u91d1", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u77e5\u5ff5, \u529f", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Yomo, Harugoro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Egawa, Yoshikazu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Miyara, Seikin", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u30aa\u30cb\u30d2\u30c8\u30c7\u80c3\u90e8\u306b\u5b58\u5728\u3059\u308b\u30b3\u30e9\u30fc\u30b2\u30ca\u30fc\u30bc, \u9178\u6027\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306f\u3044\u305a\u308c\u3082\u305d\u306e\u81f3\u9069pH\u306f2.0\u9644\u8fd1\u306b\u3042\u308b\u3002\u4e21\u9175\u7d20\u3092\u786b\u9178\u30a2\u30f3\u30e2\u30cb\u30e5\u30fc\u30e0\u6c88\u6fb1, \u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-150\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u306b\u4f9d\u308a\u5206\u96e2\u3092\u8a66\u307f\u305f\u304c\u6210\u529f\u3057\u306a\u304b\u3063\u305f\u3002\u53c8CM-\u30bb\u30eb\u30ed\u30fc\u30b9\u306b\u4f9d\u308a\u4e21\u9175\u7d20\u6d3b\u6027\u306f\u5438\u7740\u3055\u308c\u305a, DEAE-\u30bb\u30eb\u30ed\u30fc\u30b9\u306b\u4f9d\u3063\u3066\u306f\u4e21\u9175\u7d20\u3068\u3082\u82e5\u5e72\u5438\u7740\u3055\u308c, \u5438\u7740\u306e\u7a0b\u5ea6\u306b\u304a\u3044\u3066\u30b3\u30e9\u30fc\u30b2\u30ca\u30fc\u30bc\u306e\u65b9\u304c\u5f37\u304f(\u6b8a\u306bpH5.0\u306b\u304a\u3044\u3066)\u5438\u7740\u3055\u308c\u305f\u3002EDTA\u3092\u5916\u6db2\u3068\u3059\u308b\u900f\u6790\u306b\u4f9d\u308b\u4e21\u9175\u7d20\u6d3b\u6027\u306e\u5931\u6d3b\u306e\u7a0b\u5ea6, Ca^\u003c++\u003e\u30a4\u30aa\u30f3, Zn^\u003c++\u003e\u30a4\u30aa\u30f3\u306b\u4f9d\u308b\u305d\u306e\u8ce6\u6d3b\u306b\u304a\u3044\u3066\u4e21\u9175\u7d20\u306b\u304a\u3044\u3066\u8457\u3057\u3044\u5dee\u306f\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\u3002\u7136\u3057\u30b3\u30e9\u30fc\u30b2\u30ca\u30fc\u30bc\u306e\u65b9\u304c\u305d\u306e\u91d1\u5c5e\u30a4\u30aa\u30f3\u8981\u6c42\u5ea6\u306b\u304a\u3044\u3066\u5f37\u304b\u3063\u305f\u3002\u4ee5\u4e0a\u3088\u308a\u3057\u3066\u4e21\u9175\u7d20\u304c\u540c\u4e00\u30bf\u30f3\u30d1\u30af\u3067\u306f\u306a\u3044\u306b\u3057\u3066\u3082\u305d\u306e\u6027\u8cea\u304c\u8457\u3057\u304f\u4f3c\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u304b\u3068\u306a\u3063\u305f\u3002\u30da\u30d7\u30b7\u30f3\u306b\u7279\u7570\u7684\u963b\u5bb3\u5264\u306b\u3088\u308a\u30aa\u30cb\u30d2\u30c8\u30c7\u80c3\u90e8\u9178\u6027\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u304c\u963b\u5bb3\u3055\u308c\u306a\u3044\u6240\u304b\u3089, \u672c\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306f\u30da\u30d7\u30b7\u30f3\u69d8\u9175\u7d20\u3067\u306f\u306a\u304f, \u3053\u306e\u9178\u6027\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u6a19\u54c1\u306b\u542b\u307e\u308c\u308b\u30b3\u30e9\u30fc\u30b2\u30ca\u30fc\u30bc\u6d3b\u6027\u306f\u9178\u6027\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u304c\u30da\u30d7\u30b7\u30f3\u69d8\u9175\u7d20\u3067\u3042\u308b\u304c\u6545\u306e\u3082\u306e\u3067\u306a\u3044\u3053\u3068\u3092\u660e\u304b\u306b\u3057\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "Ammonium sulfate fractionation and sephadex column chromatography did not seperate collagenase and acid-protease in stomach of A. planci, both of which have an optimum pH at 2.0. Although CM-cellulose did not adsorp both enzymes, DEAE-cellulose adsorb both enzymes considerably, and more collagenase at pH 5.0. Dialysis against EDTA solution lowered both enzymes activities to the same extent and both dialysed enzyme activities were recovered by (Ca)^\u003c++\u003e and (Zn)^\u003c++\u003e completely, the collagenase being more sensitive to the metal ions than the acid-protease. These results indicate that both enzymes have strong similarity even though they are not the same protein. Since pepsin-specific inhibitors did not inhibit the acid-protease activity, the collagenase activity in the enzyme preparationdid not appears to be due to pepsin-like protease in the preparation.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4195"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. 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