{"_buckets": {"deposit": "569ac51a-eea3-4578-83a7-068a6651fdd4"}, "_deposit": {"id": "2003211", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003211"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003211", "sets": ["1642837910985", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on proteases of T. elegans venom (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u77e5\u5ff5, \u529f", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u771f\u6804\u7530, \u7fa9\u662d", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u4fdd\u826f, \u548c\u653f", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u56db\u65b9, \u6cbb\u4e94\u90ce", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Maeda, Yoshiaki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yasura, Kazumasa", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yomo, Harugoro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u6d3b\u6027\u306f, \u30ab\u30bc\u30a4\u30f3\u6d88\u5316\u6cd5\u3067\u6e2c\u5b9a\u3057\u305f\u5834\u5408, \u81f3\u9069\u6e29\u5ea6\u306f, 50\u2103\u3067\u3042\u308a, \u81f3\u9069pH\u306f, 8.0\u0026acd;9.5\u3067\u3042\u3063\u305f\u3002\u307e\u305f\u3053\u306e\u9175\u7d20\u306fpH5\u0026acd;10.5\u3067\u5b89\u5b9a\u3067\u3042\u3063\u305f\u3002\u672c\u9175\u7d20\u306f, \u30a8\u30bf\u30ce\u30fc\u30eb, \u30e1\u30bf\u30ce\u30fc\u30eb, \u30a4\u30bd\u30d7\u30ed\u30d1\u30ce\u30fc\u30eb, \u30a2\u30bb\u30c8\u30f3\u7b49\u306e\u6709\u6a5f\u6eb6\u5a92\u306b\u5bfe\u3057\u3066\u306f, \u4e0d\u5b89\u5b9a\u3067\u3042\u3063\u305f\u304c, \u786b\u5b89\u306b\u5bfe\u3057\u3066\u306f, \u5b89\u5b9a\u3067\u3042\u308b\u304c, \u9577\u6642\u9593\u653e\u7f6e\u3059\u308b\u3068\u5931\u6d3b\u3057\u305f\u3002\u307e\u305f\u5869\u5316\u30ab\u30eb\u30b7\u30a6\u30e0, \u5869\u5316\u30de\u30b0\u30cd\u30b7\u30a6\u30e0, \u5869\u5316\u4e9c\u925b\u3067\u8ce6\u6d3b\u52b9\u679c\u304c\u898b\u3089\u308c, \u7279\u306bCaCl_2\u3067\u306f, \u305d\u306e\u52b9\u679c\u304c\u5927\u3067\u3042\u3063\u305f\u3002\u672c\u9175\u7d20\u3092\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-75,G-100,G-50,\u304a\u3088\u3073DEAE\u30bb\u30eb\u30ed\u30fc\u30b9\u30ab\u30e9\u30e0\u306e\u9806\u306b\u306e\u305b\u3066\u884c\u304d\u7cbe\u88fd\u3057\u305f\u7d50\u679c, \u672c\u9175\u7d20\u306f, \u307b\u307c\u5358\u4e00\u306b\u306a\u308a, \u305d\u306e\u7d14\u5316\u7387\u306f, 3.4\u57f9\u306b\u306a\u308a, \u30bf\u30f3\u30d1\u30af\u8cea\u56de\u53ce\u7387\u306f, 4.1%\u3067\u3042\u3063\u305f\u3002\u307e\u305f\u3053\u306e\u7cbe\u88fd\u9175\u7d20\u306f, Ca^\u003c2+\u003e\u3092\u6709\u3057\u3066\u3044\u305f\u3002\u305d\u306e\u4ed6\u306b\u672c\u86c7\u6bd2\u4e2d\u306b\u306f, Zn^\u003c2+\u003e\u3092\u6709\u3059\u308b\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u304c\u5b58\u5728\u3059\u308b\u3068\u63a8\u5b9a\u3055\u308c\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "The proteinase of T. elegans venom was most active at pH8.0-9.5 and 50\u2103 with 1% casein solution as substrate, stable between pH5.0 and 10.5 and by addition of ammonium sulfate, but unstable by addition of organic solvents; ethanol, methanol, isopropanol, and acetone. The enzyme was inactivated by addition of EDTA and reactivated by CaCl_2 and MgCl_2. A proteinase was purified from the venom by gel filtration on Sephadex G-75,G-100,and G-50,and DEAE cellulose column chromatography with 4.1% over all yield and a 3.4 fold increase in specific activity. The purified enzyme was found to be homogeneous by disc gel electrophoresis and contain (Ca)^\u003c++\u003e. There was the orther proteinase in this venom, which contained (Zn)^\u003c++\u003e.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4197"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "25", "bibliographicPageEnd": "216", "bibliographicPageStart": "203"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000161951.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003211/files/KJ00000161951.pdf"}, "version_id": "0b8287ee-304a-465e-ad54-3fdbbbfe852d"}]}, "item_title": "\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837910985", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4197", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003211", "relation": {}, "relation_version_is_last": true, "title": ["\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}