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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 24号
  1. 部局別インデックス
  2. 農学部

甘蔗幹茎酸性インベルターゼに関する研究(農芸化学科)

http://hdl.handle.net/20.500.12000/4239
http://hdl.handle.net/20.500.12000/4239
10a1f7a0-2b84-43de-a00b-f95a9c012ac9
名前 / ファイル ライセンス アクション
KJ00000162597.pdf KJ00000162597.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル 甘蔗幹茎酸性インベルターゼに関する研究(農芸化学科)
言語 ja
作成者 知念, 功

× 知念, 功

ja 知念, 功

江川, 義和

× 江川, 義和

ja 江川, 義和

外間, 宏一

× 外間, 宏一

ja 外間, 宏一

四方, 治五郎

× 四方, 治五郎

ja 四方, 治五郎

Chinen, Isao

× Chinen, Isao

en Chinen, Isao

Egawa, Yoshikazu

× Egawa, Yoshikazu

en Egawa, Yoshikazu

Hokama, Koichi

× Hokama, Koichi

en Hokama, Koichi

Yomo, Harugoro

× Yomo, Harugoro

en Yomo, Harugoro

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 成長期の甘蔗幹茎搾汁より, 硫安飽和沈澱法により, 酸性インベルターゼを調製した。その調製粗酸性インベルターゼを用いて, その酵素活性測定条件を検討した。本酵素反応のpHは, 5.5であり, 温度は, 45℃であった。次にこの調製粗インベルターゼを, ゲルロ過法により精製した結果, セファデックスゲルG-150を用いて精製した場合, その活性分画の純化倍率は, 3.43倍になった。またこの活性分画をセファデックスゲルG-200を用いて精製すると, 2ツの活性ピークがみられた。その両ピークのうち, 主要活性ピークの純化倍率は, 4.78倍であった。この精製酵素を, ディスクゲル電気泳動法で, 純度を調べた結果この製精酵素では, 8ツのバンドが見られた。この精製酸性インベルターゼは, 塩化水銀, 塩化マンガン, 硫酸銅, PCMB等で著しく阻害を受けることがわかった。
内容記述タイプ Other
内容記述 Crude acid invertase was prepared from juice of sugar cane stalk by saturated ammonium salfate precipition. Assay condition for the enzyme was investigated; reaction pH was 5.5 and temparature 45℃. Two peaks of acid invertase activity were obtained by partitial purification from the crude enzyme by Sephadex G-150 and G-200 gel colume chromatography and specific activity of the main active peak was 4.78 fold increase. The acid invertase purified revealed eight bands by poly acrylamide disc gel electropholesis and were significantly inactivated by HgCl_2,MnCl_2,CuSO_4 and PCMB.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4239
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 24, p. 217-229
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