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  1. その他
  1. 部局別インデックス
  2. 医学部

良性卵巣奇形腫を利用したヒトにおける配偶子特異的メチル化機構の解析

http://hdl.handle.net/20.500.12000/14310
http://hdl.handle.net/20.500.12000/14310
d691cbad-c87e-4527-b964-4d8986fc8591
名前 / ファイル ライセンス アクション
10672138.pdf 10672138.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2009-12-25
タイトル
タイトル 良性卵巣奇形腫を利用したヒトにおける配偶子特異的メチル化機構の解析
言語 ja
作成者 陣野, 吉廣

× 陣野, 吉廣

ja 陣野, 吉廣

石丸, 忠之

× 石丸, 忠之

ja 石丸, 忠之

Jinno, Yoshihiro

× Jinno, Yoshihiro

en Jinno, Yoshihiro

Ishimaru, Tadayuki

× Ishimaru, Tadayuki

en Ishimaru, Tadayuki

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
主題
言語 ja
主題Scheme Other
主題 良性卵巣奇形腫
言語 ja
主題Scheme Other
主題 インプリンティング
言語 ja
主題Scheme Other
主題 メチレーション
言語 ja
主題Scheme Other
主題 内在性レトロウィルス
内容記述
内容記述タイプ Other
内容記述 科研費番号: 10672138
内容記述タイプ Other
内容記述 平成10年度~平成11年度科学研究費補助金(基盤研究(C)(2))研究成果報告書
内容記述タイプ Other
内容記述 研究概要:刷り込み遺伝子の配偶子特異的メチル化機構を探るため、良性卵巣奇形腫(以下、奇形腫)を利用した。最初に45のDNAマーカーで25例の奇形腫の分類を行った。メチル化解析は父性メチル化の代表としてH19、母性メチル化の代表としてSNRPNを選んでサザーン法およびPCR法により行った。また、ヒト内在性レトロウィルス(HERV)K-familyを選び、活性別およびインプリンティングドメイン内外の局在別に解析することを計画した。結果および結論を以下に要約する。1.欧米の報告と異なり、meiosisIIからの奇形腫発生は25例中1例(4%)と稀である。2.奇形腫は遺伝的に異質な(heterogenousu)細胞集団から成る。3.個々の奇形腫におけるH19およびSNRPNのメチル化はきれいな逆相関関係にあり、奇形腫発生の卵形成上におけるステージが進むにつれ、両者のメチル化パターンは卵子のそれに近づく。4.ヒトH19のprimary imprintは卵形成のかなり遅い時期まで完全に消去されない。またSNRPNのprimary imprintも同様に遅くまで確立されない。5.以上より、配偶子特異的primary imprintの形成は漸進的構築過程であること、primary imprintの消去および確立と相反する二つの機構になんらかの共役した過程が存在することが示唆される。6.胎盤、精巣、肺および末血リンパ球で6種類のHERV-Kの発現が認められ、複数の組織で発現していた3つの活性型HERV-Kを単離したが、11p15.5および15q11-q13に局在するHERV-Kはコスミドライブラリーのスクリーニングからもコンピューター検索からも得ることができなかった。
内容記述タイプ Other
内容記述 要約(欧文):In humans, studies of female germ cells are very limited by ethics. The current study investigated the usefulness of benign ovarian teratomas as a substitute for ova in analyses of imprinted genes. Twenty-five human benign ovarian teratomas were typed with 45 microsatellite DNA markers and classified according to their genotypic features. Two oppositely imprinted genes, H19 and SNRPN, were then chosen for analysis of their methylation states in these tumors. These analyses revealed that benign ovarian teratomas consist of a mixture of genetically and epigenetically heterogeneous cell populations. In contrast to previous reports, we could document only one case rising from germ cells by meiosis-II nondisjunction. H19 and SNRPN were methylated in individual teratomas to various degrees, ranging from normal somatic cell to expected ovum levels. The allele with residual methylation of H19 was consistent with that methylated in the patient's blood DNA, thus being of paternal origin. Degrees of H19 hypomethylation and SNRPN hypermethylation increased as the cellular origin of the tumors advanced in oogenesis and were closely correlated in individual teratomas. These results could be best explained by the assumption that the primary imprinting is a progressively organized process and suggest that the establishment of primary imprints on different genes might be mechanistically linked, even when those genes are oppositely imprinted. Transcripts of six genes of human endogenous retrovirus K (HERV-K)-family members were detected by sequencing of RT-PCR clones in the placenta, testis, peripheral blood leukocytes and fetal lung. Three of them, which were expressed at least in the two tissues analysed, were isolated from a BAC library.
内容記述タイプ Other
内容記述 未公開:P.7以降(別刷論文のため)
内容記述タイプ Other
内容記述 研究報告書
出版者
言語 ja
出版者 陣野吉廣
言語
言語 jpn
資源タイプ
資源タイプ research report
資源タイプ識別子 http://purl.org/coar/resource_type/c_18ws
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/14310
識別子タイプ HDL
収録物識別子
収録物識別子タイプ NCID
収録物識別子 BA46530571
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