Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2010-01-04 |
タイトル |
|
|
タイトル |
Helicobacter pylori-Induced Interleukin-12 p40 Expression |
|
言語 |
en |
作成者 |
Takeshima, Eriko
Tomimori, Koh
Teruya, Hiromitsu
Ishikawa, Chie
Senba, Masachika
D' Ambrosio, Daniele
Kinjo, Fukunori
Mimuro, Hitomi
Sasakawa, Chihiro
Hirayama, Toshiya
Fujita, Jiro
Mori, Naoki
|
アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
|
|
言語 |
en |
|
権利情報 |
Copyright (c) 2009, American Society for Microbiology. All Rights Reserved. |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Interleukin-12 (IL-12) is a heterodimeric cytokine produced by antigen-presenting cells that promotes the development of T-helper lymphocyte 1 (Th1). Chronic gastritis induced by Helicobacter pylori is considered a Th1-mediated process. IL-12 levels in gastric biopsy samples of H. pylori-infected patients are higher than in those of uninfected individuals, but the cellular source of IL-12 remains elusive. IL-12 staining was detected in mucosal epithelial cells, lymphocytes, and macrophages in specimens of patients with H. pylori-positive gastritis. Therefore, we investigated IL-12 p40 mRNA induction by H. pylori in gastric epithelial cells and T cells. Although cag pathogenicity island (PAI)-positive H. pylori induced IL-12 p40 mRNA expression, an isogenic mutant of the cag PAI failed to induce it in both cell types. Supernatants from H. pylori cultures and H. pylori VacA induced IL-12 p40 mRNA expression in T cells but not in epithelial cells. The activation of the IL-12 p40 promoter by H. pylori was mediated through NF-κB. The transfection of IκB kinase and NF-κB-inducing kinase dominant-negative mutants inhibited H. pylori-induced IL-12 p40 activation. Inhibitors of NF-κB, phosphatidylinositol 3-kinase, p38 mitogen-activated protein kinase, and Hsp90 suppressed H. pylori- and VacA-induced IL-12 p40 mRNA expression. The results indicate that H. pylori induces IL-12 p40 expression by the activation of NF-κB, phosphatidylinositol 3-kinase, and p38 mitogen-activated protein kinase. Hsp90 is also a crucial regulator of H. pylori-induced IL-12 p40 expression. In addition to the cag PAI, VacA might be relevant in the induction of IL-12 expression and a Th1-polarized response only in T cells. |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
出版者 |
|
|
言語 |
en |
|
出版者 |
American Society for Microbiology |
言語 |
|
|
言語 |
eng |
資源タイプ |
|
|
資源タイプ |
journal article |
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
識別子 |
|
|
識別子 |
http://hdl.handle.net/20.500.12000/14407 |
|
識別子タイプ |
HDL |
関連情報 |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://iai.asm.org/cgi/content/abstract/77/4/1337 |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1128/IAI.01456-08 |
収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0019-9567 |
収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00673732 |
収録物名 |
|
|
言語 |
en |
|
収録物名 |
Infection and immunity |
書誌情報 |
巻 77,
号 4,
p. 1337-1348,
発行日 2009-04
|