{"_buckets": {"deposit": "9c27f420-56e5-44d3-b630-4c703681efd1"}, "_deposit": {"id": "2005221", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2005221"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02005221", "sets": ["1642838338003", "1642838407795"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Helicobacter pylori-Induced Interleukin-12 p40 Expression", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takeshima, Eriko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tomimori, Koh", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Teruya, Hiromitsu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Chie", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Senba, Masachika", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "D\u0027 Ambrosio, Daniele", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kinjo, Fukunori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Mimuro, Hitomi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sasakawa, Chihiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hirayama, Toshiya", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Fujita, Jiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Mori, Naoki", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "en", "subitem_1522651041219": "Copyright (c) 2009, American Society for Microbiology. All Rights Reserved."}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Interleukin-12 (IL-12) is a heterodimeric cytokine produced by antigen-presenting cells that promotes the development of T-helper lymphocyte 1 (Th1). Chronic gastritis induced by Helicobacter pylori is considered a Th1-mediated process. IL-12 levels in gastric biopsy samples of H. pylori-infected patients are higher than in those of uninfected individuals, but the cellular source of IL-12 remains elusive. IL-12 staining was detected in mucosal epithelial cells, lymphocytes, and macrophages in specimens of patients with H. pylori-positive gastritis. Therefore, we investigated IL-12 p40 mRNA induction by H. pylori in gastric epithelial cells and T cells. Although cag pathogenicity island (PAI)-positive H. pylori induced IL-12 p40 mRNA expression, an isogenic mutant of the cag PAI failed to induce it in both cell types. Supernatants from H. pylori cultures and H. pylori VacA induced IL-12 p40 mRNA expression in T cells but not in epithelial cells. The activation of the IL-12 p40 promoter by H. pylori was mediated through NF-\u03baB. The transfection of I\u03baB kinase and NF-\u03baB-inducing kinase dominant-negative mutants inhibited H. pylori-induced IL-12 p40 activation. Inhibitors of NF-\u03baB, phosphatidylinositol 3-kinase, p38 mitogen-activated protein kinase, and Hsp90 suppressed H. pylori- and VacA-induced IL-12 p40 mRNA expression. The results indicate that H. pylori induces IL-12 p40 expression by the activation of NF-\u03baB, phosphatidylinositol 3-kinase, and p38 mitogen-activated protein kinase. Hsp90 is also a crucial regulator of H. pylori-induced IL-12 p40 expression. In addition to the cag PAI, VacA might be relevant in the induction of IL-12 expression and a Th1-polarized response only in T cells.", "subitem_description_type": "Other"}, {"subitem_description": "\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "en", "subitem_1522300316516": "American Society for Microbiology"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/14407"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0019-9567"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AA00673732"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "Infection and immunity"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2009-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "1348", "bibliographicPageStart": "1337", "bibliographicVolumeNumber": "77"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "AM", "subitem_1600292170262": "http://purl.org/coar/version/c_ab4af688f83e57aa"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "URI", "subitem_1522306436033": "http://iai.asm.org/cgi/content/abstract/77/4/1337"}}, {"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "10.1128/IAI.01456-08"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "IAI01456-08R2FIG.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2005221/files/IAI01456-08R2FIG.pdf"}, "version_id": "6cb4692b-35a4-4df0-9b9d-f245e2b28f7d"}, {"accessrole": "open_access", "download_preview_message": "", "file_order": 1, "filename": "IAI01456-08R2.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2005221/files/IAI01456-08R2.pdf"}, "version_id": "5bda5446-b585-41ab-a9bc-d86fbb08cacf"}]}, "item_title": "Helicobacter pylori-Induced Interleukin-12 p40 Expression", "item_type_id": "15", "owner": "1", "path": ["1642838338003", "1642838407795"], "permalink_uri": "http://hdl.handle.net/20.500.12000/14407", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-01-04"}, "publish_date": "2010-01-04", "publish_status": "0", "recid": "2005221", "relation": {}, "relation_version_is_last": true, "title": ["Helicobacter pylori-Induced Interleukin-12 p40 Expression"], "weko_shared_id": -1}