Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2018-01-18 |
タイトル |
|
|
タイトル |
Cirsium brevicaule A. GRAY leaf inhibits adipogenesis in 3T3-L1 cells and C57BL/6 mice |
|
言語 |
en |
作成者 |
Inafuku, Masashi
Ruwani N, Nugara
Kamiyama, Yasuo
Futenma, Itsuki
Inafuku, Ayako
Oku, Hirosuke
|
アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cirsium brevicaule A. GRAY |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Anti-adipogenesis |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Obesity |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Non-alcoholic fatty liver disease |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Background: Various flavonoids obtained from the genus Cirsium have been reported to exhibit beneficial effects on health. The present study evaluated the antiobesity effects of Cirsium brevicaule A. GRAY leaf (CL) by using 3T3-L1 cells and C57BL/6 mice that were fed a high-fat diet (HFD). Methods: Dried CL powder was serially extracted with solvents of various polarities, and these extracts were tested for antiadipogenic activity using 3T3-L1 adipocytes. Mice were fed experimental HFD supplemented with dried CL powder for 4 wk. Lipid levels and mRNA levels of genes related to lipid metabolism were determined in 3T3-L1 adipocytes and the white adipose tissue (WAT) and liver of mice fed on a HFD. Results: Treatment of 3T3-L1 adipocytes with a hexane extract of CL significantly reduced cellular lipid accumulation and expression of the fatty acid synthase (FASN) gene. Dietary CL reduced the serum levels of nonesterified fatty acids in HFD-fed mice. Significant decreases in subcutaneous WAT weight and associated FASN gene expression were observed in the mice fed the experimental CL diet. Dietary CL also reduced the hepatic lipid and\nserum levels of a hepatopathic indicator in the HFD-fed mice. A significant reduction in mRNA levels of FASN and HMG-CoA reductase were observed in the livers of the CL-diet group. Dietary CL, on the other hand, increased in the hepatic mRNA levels of genes related to β-oxidation, namely peroxisome proliferator-activated receptor α, calnitine palmitoyltrasferase 1A, and uncoupling protein 2. Expression of the insulin receptor gene was also significantly increased in the livers of mice-fed the CL diet. Conclusions: The present study therefore demonstrated that CL suppresses lipid accumulation in the WAT and liver partly through inhibiting mRNA levels of FASN gene and enhancing the lipolysis-related gene expression. |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
出版者 |
|
|
言語 |
en |
|
出版者 |
BioMed Central |
言語 |
|
|
言語 |
jpn |
資源タイプ |
|
|
資源タイプ |
journal article |
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
識別子 |
|
|
識別子 |
http://hdl.handle.net/20.500.12000/37587 |
|
識別子タイプ |
HDL |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1186/1476-511X-12-124 |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1186/1476-511X-12-124 |
関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
23945333 |
収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1476-511X |
収録物名 |
|
|
言語 |
en |
|
収録物名 |
Lipids in Health and Disease |
書誌情報 |
巻 12,
p. 124-132,
発行日 2013-08-15
|