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Duplication and soldier-specific expression of geranylgeranyl diphosphate synthase genes in a nasute termite Nasutitermes takasagoensis
http://hdl.handle.net/20.500.12000/47405
http://hdl.handle.net/20.500.12000/47405582bb494-adec-41e2-903d-7cf4ee775581
| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2020-12-04 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Duplication and soldier-specific expression of geranylgeranyl diphosphate synthase genes in a nasute termite Nasutitermes takasagoensis | |||||||||||||
| 言語 | en | |||||||||||||
| 作成者 |
Hojo, Masaru
× Hojo, Masaru
× Shigenobu, Shuji
× Maekawa, Kiyoto
× Miura, Toru
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| アクセス権 | ||||||||||||||
| アクセス権 | open access | |||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Chemical defence | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Exocrine gland | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Mevalonate pathway | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Nasus | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Termitidae | |||||||||||||
| 内容記述 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | In the evolutionarily-derived termite subfamily Nasutitermitinae (family Termitidae), soldiers defend their nestmates by discharging polycyclic diterpenes from a head projection called the “nasus.” The diterpenes are synthesised in the frontal gland from the precursor geranylgeranyl diphosphate (GGPP), which is generally used for post-translational modification of proteins in animals. In this study, we constructed a comprehensive gene catalogue to search for genes involved in the diterpene biosynthesis by assembling RNA sequencing reads of Nasutitermes takasagoensis, identifying eight gene copies for GGPP synthase (GGPPS). The number of gene copies is much larger in contrast to other related insects. Gene cloning by reverse transcription-PCR and rapid amplification of cDNA ends confirmed that seven GGPPS genes (NtGGPPS1 to NtGGPPS7) have highly variable untranslated regions. Molecular phylogenetic analysis showed that theNtGGPPS7gene was grouped with homologs obtained from ancestral termites that have only a single copy of the gene, and theNtGGPPS6gene was grouped with homologs obtained from a basal lineage of termitids, in which soldiers do not synthesise diterpenes. As the sister group to this clade, furthermore, a monophyletic clade included all the other NtGGPPS genes (NtGGPPS1toNtGGPPS5). Expression analyses revealed thatNtGGPPS7gene was expressed in all the examined castes and tissues, whereas all the other genes were expressed only in the soldier head. These results suggest that gene duplication followed by subfunctionalisation of the GGPPS genes might have accompanied the evolution of chemical defence in the nasute termite lineage. | |||||||||||||
| 内容記述 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | 論文 | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | Elsevier | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | AM | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
| 識別子 | ||||||||||||||
| 識別子 | http://hdl.handle.net/20.500.12000/47405 | |||||||||||||
| 識別子タイプ | HDL | |||||||||||||
| 関連情報 | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.ibmb.2019.103177 | |||||||||||||
| 関連情報 | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.ibmb.2019.103177 | |||||||||||||
| 関連情報 | ||||||||||||||
| 識別子タイプ | PMID | |||||||||||||
| 関連識別子 | 31228516 | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0965-1748 | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1879-0240 | |||||||||||||
| 収録物名 | ||||||||||||||
| 収録物名 | Insect Biochemistry and Molecular Biology | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
巻 111, 発行日 2019-06-20 |
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