{"_buckets": {"deposit": "14ce71bf-b086-4651-81dd-8b20012f12b1"}, "_deposit": {"id": "2012290", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2012290"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02012290", "sets": ["1642838338003", "1642838407795"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Shibata, Yuri", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tokunaga, Fuminori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Goto, Eiji", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Komatsu, Ginga", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Gohda, Jin", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Saeki, Yasushi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tanaka, Keiji", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Takahashi, Hirotaka", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sawasaki, Tatsuya", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Inoue, Satoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Oshiumi, Hiroyuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Seya, Tsukasa", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nakano, Hiroyasu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tanaka, Yuetsu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Iwai, Kazuhiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Inoue, Jun-ichiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "en", "subitem_1522651041219": "Creative Commons Attribution License 4.0"}, {"subitem_1522650717957": "en", "subitem_1522650727486": "https://creativecommons.org/licenses/by/4.0/", "subitem_1522651041219": "https://creativecommons.org/licenses/by/4.0/"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "The Tax protein of human T-cell leukemia virus type 1 (HTLV-1) is crucial for the development of adult T-cell leukemia (ATL), a highly malignant CD4+ T cell neoplasm. Among the multiple aberrant Tax-induced effects on cellular processes, persistent activation of transcription factor NF-κB, which is activated only transiently upon physiological stimulation, is essential for leukemogenesis. We and others have shown that Tax induces activation of the IκB kinase (IKK) complex, which is a critical step in NF-κB activation, by generating Lys63- linked polyubiquitin chains. However, the molecular mechanism underlying Tax-induced IKK activation is controversial and not fully understood. Here, we demonstrate that Tax recruits linear (Met1-linked) ubiquitin chain assembly complex (LUBAC) to the IKK complex and that Tax fails to induce IKK activation in cells that lack LUBAC activity. Mass spectrometric analyses revealed that both Lys63-linked and Met1-linked polyubiquitin chains are associated with the IKK complex. Furthermore, treatment of the IKK-associated polyubiquitin chains with Met1-linked-chain-specific deubiquitinase (OTULIN) resulted in the reduction of high molecular weight polyubiquitin chains and the generation of short Lys63-linked ubiquitin chains, indicating that Tax can induce the generation of Lys63- and Met1-linked hybrid polyubiquitin chains. We also demonstrate that Tax induces formation of the active macromolecular IKK complex and that the blocking of Tax-induced polyubiquitin chain synthesis inhibited formation of the macromolecular complex. Taken together, these results lead us to propose a novel model in which the hybrid-chain-dependent oligomerization of the IKK complex triggered by Tax leads to trans-autophosphorylation-mediated IKK activation.", "subitem_description_type": "Other"}, {"subitem_description": "論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "en", "subitem_1522300316516": "Public Library of Science"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/45642"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1553-7374"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "PLOS Pathogens"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-01-19", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicVolumeNumber": "13"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1371/journal.ppat.1006162"}}, {"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1371/journal.ppat.1006162"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "journal.ppat.1006162.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2012290/files/journal.ppat.1006162.pdf"}, "version_id": "643bff3c-d2fc-4fe4-abc3-abf3cdb895e4"}]}, "item_title": "HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains", "item_type_id": "15", "owner": "1", "path": ["1642838338003", "1642838407795"], "permalink_uri": "http://hdl.handle.net/20.500.12000/45642", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2020-04-15"}, "publish_date": "2020-04-15", "publish_status": "0", "recid": "2012290", "relation": {}, "relation_version_is_last": true, "title": ["HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains"], "weko_shared_id": -1}