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Both plus- and minus-strands of the provirus are transcribed, respectively from the 5\u2032 and 3\u2032 long terminal repeats (LTR). Plus-strand expression is rapid and intense once activated, whereas the minus-strand is transcribed at a lower, more constant level. To identify how HTLV-1 transcription is regulated, we investigated the epigenetic modifications associated with the onset of spontaneous plus-strand expression and the potential impact of the host factor CTCF.\\nMethods: Patient-derived peripheral blood mononuclear cells (PBMCs) and in vitro HTLV-1-infected T cell clones were examined. Cells were stained for the plus-strand-encoded viral protein Tax, and sorted into Tax+ and Tax\u2013 populations. Chromatin immunoprecipitation and methylated DNA immunoprecipitation were performed to identify epigenetic modifications in the provirus. Bisulfite-treated DNA fragments from the HTLV-1 LTRs were sequenced. 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  1. 学術雑誌論文
  2. その他
  1. 部局別インデックス
  2. 医学部

Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent

http://hdl.handle.net/20.500.12000/46085
http://hdl.handle.net/20.500.12000/46085
0cb95d51-2d4a-44b7-bafb-f6fd9043c076
名前 / ファイル ライセンス アクション
b03c4970-e75a-409a-b692-d05c9b7c59a2_14741_-_charles_bangham_v2.pdf b03c4970-e75a-409a-b692-d05c9b7c59a2_14741_-_charles_bangham_v2.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2020-06-09
タイトル
タイトル Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent
言語 en
作成者 Miura, Michi

× Miura, Michi

en Miura, Michi

Miyazato, Paola

× Miyazato, Paola

en Miyazato, Paola

Satou, Yorifumi

× Satou, Yorifumi

en Satou, Yorifumi

Tanaka, Yuetsu

× Tanaka, Yuetsu

en Tanaka, Yuetsu

Bangham, Charles R.M.

× Bangham, Charles R.M.

en Bangham, Charles R.M.

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 en
権利情報 Creative Commons Attribution 4.0
言語 en
権利情報Resource https://creativecommons.org/licenses/by/4.0/
権利情報 https://creativecommons.org/licenses/by/4.0/
主題
言語 en
主題Scheme Other
主題 Histone modifications
言語 en
主題Scheme Other
主題 DNA methylation
言語 en
主題Scheme Other
主題 Epigenetics
言語 en
主題Scheme Other
主題 Antisense transcription
言語 en
主題Scheme Other
主題 Transcription kinetics
言語 en
主題Scheme Other
主題 Retrovirus
言語 en
主題Scheme Other
主題 HTLV-1
言語 en
主題Scheme Other
主題 CTCF
言語 en
主題Scheme Other
主題 CRISPR/Cas9
言語 en
主題Scheme Other
主題 Single-molecule RNA-FISH
内容記述
内容記述タイプ Other
内容記述 Background: The human retrovirus HTLV-1 inserts the viral complementary DNA of 9 kb into the host genome. Both plus- and minus-strands of the provirus are transcribed, respectively from the 5′ and 3′ long terminal repeats (LTR). Plus-strand expression is rapid and intense once activated, whereas the minus-strand is transcribed at a lower, more constant level. To identify how HTLV-1 transcription is regulated, we investigated the epigenetic modifications associated with the onset of spontaneous plus-strand expression and the potential impact of the host factor CTCF.\nMethods: Patient-derived peripheral blood mononuclear cells (PBMCs) and in vitro HTLV-1-infected T cell clones were examined. Cells were stained for the plus-strand-encoded viral protein Tax, and sorted into Tax+ and Tax– populations. Chromatin immunoprecipitation and methylated DNA immunoprecipitation were performed to identify epigenetic modifications in the provirus. Bisulfite-treated DNA fragments from the HTLV-1 LTRs were sequenced. Single-molecule RNA-FISH was performed, targeting HTLV-1 transcripts, for the estimation of transcription kinetics. The CRISPR/Cas9 technique was applied to alter the CTCF-binding site in the provirus, to test the impact of CTCF on the epigenetic modifications.\nResults: Changes in the histone modifications H3K4me3, H3K9Ac and H3K27Ac were strongly correlated with plus-strand expression. DNA in the body of the provirus was largely methylated except for the pX and 3′ LTR regions, regardless of Tax expression. The plus-strand promoter was hypomethylated when Tax was expressed. Removal of CTCF had no discernible impact on the viral transcription or epigenetic modifications.\nConclusions: The histone modifications H3K4me3, H3K9Ac and H3K27Ac are highly dynamic in the HTLV-1 provirus: they show rapid change with the onset of Tax expression, and are reversible. The HTLV-1 provirus has an intrinsic pattern of epigenetic modifications that is independent of both the provirus insertion site and the chromatin architectural protein CTCF which binds to the HTLV-1 provirus.
内容記述タイプ Other
内容記述 論文
出版者
言語 en
出版者 F1000Research
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/46085
識別子タイプ HDL
関連情報
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.12688/wellcomeopenres.14741.2
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.12688/wellcomeopenres.14741.2
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 2398-502X
収録物名
言語 en
収録物名 Wellcome Open Research
書誌情報
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