Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2020-06-09 |
タイトル |
|
|
タイトル |
Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent |
|
言語 |
en |
作成者 |
Miura, Michi
Miyazato, Paola
Satou, Yorifumi
Tanaka, Yuetsu
Bangham, Charles R.M.
|
アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
|
|
言語 |
en |
|
権利情報 |
Creative Commons Attribution 4.0 |
権利情報 |
|
|
言語 |
en |
|
権利情報Resource |
https://creativecommons.org/licenses/by/4.0/ |
|
権利情報 |
https://creativecommons.org/licenses/by/4.0/ |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Histone modifications |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA methylation |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Epigenetics |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Antisense transcription |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Transcription kinetics |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Retrovirus |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
HTLV-1 |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CTCF |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CRISPR/Cas9 |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Single-molecule RNA-FISH |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Background: The human retrovirus HTLV-1 inserts the viral complementary DNA of 9 kb into the host genome. Both plus- and minus-strands of the provirus are transcribed, respectively from the 5′ and 3′ long terminal repeats (LTR). Plus-strand expression is rapid and intense once activated, whereas the minus-strand is transcribed at a lower, more constant level. To identify how HTLV-1 transcription is regulated, we investigated the epigenetic modifications associated with the onset of spontaneous plus-strand expression and the potential impact of the host factor CTCF.\nMethods: Patient-derived peripheral blood mononuclear cells (PBMCs) and in vitro HTLV-1-infected T cell clones were examined. Cells were stained for the plus-strand-encoded viral protein Tax, and sorted into Tax+ and Tax– populations. Chromatin immunoprecipitation and methylated DNA immunoprecipitation were performed to identify epigenetic modifications in the provirus. Bisulfite-treated DNA fragments from the HTLV-1 LTRs were sequenced. Single-molecule RNA-FISH was performed, targeting HTLV-1 transcripts, for the estimation of transcription kinetics. The CRISPR/Cas9 technique was applied to alter the CTCF-binding site in the provirus, to test the impact of CTCF on the epigenetic modifications.\nResults: Changes in the histone modifications H3K4me3, H3K9Ac and H3K27Ac were strongly correlated with plus-strand expression. DNA in the body of the provirus was largely methylated except for the pX and 3′ LTR regions, regardless of Tax expression. The plus-strand promoter was hypomethylated when Tax was expressed. Removal of CTCF had no discernible impact on the viral transcription or epigenetic modifications.\nConclusions: The histone modifications H3K4me3, H3K9Ac and H3K27Ac are highly dynamic in the HTLV-1 provirus: they show rapid change with the onset of Tax expression, and are reversible. The HTLV-1 provirus has an intrinsic pattern of epigenetic modifications that is independent of both the provirus insertion site and the chromatin architectural protein CTCF which binds to the HTLV-1 provirus. |
内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
出版者 |
|
|
言語 |
en |
|
出版者 |
F1000Research |
言語 |
|
|
言語 |
eng |
資源タイプ |
|
|
資源タイプ |
journal article |
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
識別子 |
|
|
識別子 |
http://hdl.handle.net/20.500.12000/46085 |
|
識別子タイプ |
HDL |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.12688/wellcomeopenres.14741.2 |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.12688/wellcomeopenres.14741.2 |
収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2398-502X |
収録物名 |
|
|
言語 |
en |
|
収録物名 |
Wellcome Open Research |
書誌情報 |
巻 3,
発行日 2018-12-11
|