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The transplanted pellets were covered with fibrin glue (pellet  group) , whereas the osteochondral defect filled only with fibrin glue was used as a control  (control group). At 4, 8, 16, 24 weeks after the treatment, we performed macroscopic comparison,  histological evaluation (toluidine blue, Mankin\u0027s score) and fluorescence in situ  hybridization (FISH) of human X and Y chromosomes. The macroscopic findings of the  pellet group at 24 weeks showed shiny and smooth surface, whereas the control group  showed rough surface and subsided. After the treatment of osteochondral defect with pellets,  the depth of the defect became thinner sequentially but surface layer consisted of hyaline like cartilage histologically. On the other hands, the surface of the defect treated without  pellets was fibrous tissues. 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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 27巻3・4号
  1. 部局別インデックス
  2. その他

[原著]Repair of Osteochondral Defects in Rat Knees with HumanBone Marrow Mesenchymal Stem Cell

http://hdl.handle.net/20.500.12000/0002015584
http://hdl.handle.net/20.500.12000/0002015584
2f353461-1ba0-4ded-9ace-a2268bf30fdf
名前 / ファイル ライセンス アクション
v27p115.pdf v27p115.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2010-02-23
タイトル
タイトル [原著]Repair of Osteochondral Defects in Rat Knees with HumanBone Marrow Mesenchymal Stem Cell
言語 ja
作成者 Hayashi, Kaori

× Hayashi, Kaori

en Hayashi, Kaori

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Kanaya, Fminori

× Kanaya, Fminori

en Kanaya, Fminori

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Fu, Freddy H

× Fu, Freddy H

en Fu, Freddy H

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 mesenchymal
主題
言語 en
主題Scheme Other
主題 stem cell
主題
言語 en
主題Scheme Other
主題 osteochondral defect
主題
言語 en
主題Scheme Other
主題 cartilage repair
主題
言語 en
主題Scheme Other
主題 pellet culture
主題
言語 en
主題Scheme Other
主題 transplantation
内容記述
内容記述タイプ Other
内容記述 Articular cartilage (hyaline cartilage) has only a limited capacity for intrinsic healing. Human mesenchymal stem cells (MSCs) from bone marrow are useful candidate for cell sources in tissue engineering, since MSCs have the capacity to differentiate into various tissues. In this study, the pellets containing hyaline like cartilage differentiated from MSCs were transplanted in rat knee joints in an attempt to repair full-thickness osteochondral defect. MSCs, which were obtained by isolating from human bone marrow, were centrifuged and the resultant pellet was cultured in a medium containing various concentration of TGFbeta1 in the presence or absence of fetal bovine serum (FBS) for 2 weeks. As the results, the pellets cultured under a condition with 10 ng/ml TGF-beta1 and absence of FBS contained significant hyaline like cartilage that were evaluated histologically. The pellets cultured under this condition were transplanted into the osteochondral defect in the patellar groove of athymic rat knee joints. The transplanted pellets were covered with fibrin glue (pellet group) , whereas the osteochondral defect filled only with fibrin glue was used as a control (control group). At 4, 8, 16, 24 weeks after the treatment, we performed macroscopic comparison, histological evaluation (toluidine blue, Mankin's score) and fluorescence in situ hybridization (FISH) of human X and Y chromosomes. The macroscopic findings of the pellet group at 24 weeks showed shiny and smooth surface, whereas the control group showed rough surface and subsided. After the treatment of osteochondral defect with pellets, the depth of the defect became thinner sequentially but surface layer consisted of hyaline like cartilage histologically. On the other hands, the surface of the defect treated without pellets was fibrous tissues. The Mankin's score showed no significant differences among weeks, however, the score of the pellet group at 24 weeks was significantly lower than that of the control group, suggesting that the histological repair of the pellet group was better than that of the control group. At 2 and 8 weeks after the transplantation, human cells were detected in the transplanted place but were not observed at 16 weeks by FISH. Although we could not explain the role of the transplanted cells, the full-thickness cartilage defect treated with pellets showed macroscopically nearly normal, and the surface of the defect was covered with hyaline like cartilage. These results suggest that the transplantation of pellets containing hyaline like cartilage differentiated from MSCs may have potential to restore full-thickness osteochondral defect.
内容記述
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
出版者
言語 en
出版者 Ryukyu Medical Association
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346-888X
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0289-1530
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 27, 号 3・4, p. 115-122, 発行日 2008
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