{"_buckets": {"deposit": "6cf23414-0b35-47e3-a50e-0f6c0b77bb31"}, "_deposit": {"id": "2015734", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2015734"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02015734", "sets": ["1642838234333", "1642838412624"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "[\u539f\u8457\uff3dEstimation of Sodium- Potassium ATPase Activity of Skeletal Muscle Hemogenate and Washed Particle", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takeo, Satoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sakanashi, Matao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Aniya, Yoko", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "ja", "subitem_1522651041219": "\u7409\u7403\u533b\u5b66\u4f1a"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Sodium-potassium ATPase"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Ouabain"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Red muscle"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Vanadium"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "White muscle"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Applicability of ouabain and vanadium for estimation of Na-K ATPase of crude fractions of skeletal red and white muscles was examined. More than 3 mM ouabain or 300 nM vanadium significantly reduced magnesium-activated ATPase activity of skeletal muscle homogenate and washed particle. Na-K ATPase activity of red muscle homogenate estimated using 1 mM ouabain was about 2-fold higher than that of the washed particle, while the Na-K ATPase activity of white muscle homogenate was about half in comparison with that of the washed particle. This indicates differences in changes of Na-K ATPase activity between skeletal red and white muscles during preparation of washed particle from the homogenate. Na-K ATPase activity of skeletal muscle homogenate estimated using 100 nM vanadium showed a similar activity to that of the washed particle, which was lower than that estimated by 1 mM ouabain. The present results suggest applicability of ouabain as a tool for estimation of Na-K ATPase in skeletal muscle crude fraction.", "subitem_description_type": "Other"}, {"subitem_description": "\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u533b\u5b66\u90e8"}, {"subitem_1522300295150": "en", "subitem_1522300316516": "Faculty of Medicine, University of the Ryukyus"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0289-1530"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN10027430"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u533b\u5b66\u4f1a\u96d1\u8a8c : \u533b\u5b66\u90e8\u7d00\u8981 = Ryukyu medical journal"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "3-4", "bibliographicPageEnd": "166", "bibliographicPageStart": "158", "bibliographicVolumeNumber": "6"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "v6p158.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2015734/files/v6p158.pdf"}, "version_id": "1b36df15-656b-4f97-a105-1e21c61e7c7a"}]}, "item_title": "[\u539f\u8457\uff3dEstimation of Sodium- Potassium ATPase Activity of Skeletal Muscle Hemogenate and Washed Particle", "item_type_id": "15", "owner": "1", "path": ["1642838234333", "1642838412624"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002015734", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-06-30"}, "publish_date": "2010-06-30", "publish_status": "0", "recid": "2015734", "relation": {}, "relation_version_is_last": true, "title": ["[\u539f\u8457\uff3dEstimation of Sodium- Potassium ATPase Activity of Skeletal Muscle Hemogenate and Washed Particle"], "weko_shared_id": -1}