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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 17巻1号
  1. 部局別インデックス
  2. その他

[原著]Graft-directed gene transfection in a rat model of liver transplantation

http://hdl.handle.net/20.500.12000/0002016118
http://hdl.handle.net/20.500.12000/0002016118
9bd15467-d410-4ec5-9591-835e73dd1035
名前 / ファイル ライセンス アクション
v17p21.pdf v17p21.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2010-07-02
タイトル
タイトル [原著]Graft-directed gene transfection in a rat model of liver transplantation
言語 ja
作成者 Shiraishi, Masayuki

× Shiraishi, Masayuki

en Shiraishi, Masayuki

Hiroyasu, Shungo

× Hiroyasu, Shungo

en Hiroyasu, Shungo

Tomori, Hirohumi

× Tomori, Hirohumi

en Tomori, Hirohumi

Ma, Shao-ping

× Ma, Shao-ping

en Ma, Shao-ping

Makino, Yoshihiro

× Makino, Yoshihiro

en Makino, Yoshihiro

Kusano, Toshiomi

× Kusano, Toshiomi

en Kusano, Toshiomi

Muto, Yoshihiro

× Muto, Yoshihiro

en Muto, Yoshihiro

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 gene transfer
言語 en
主題Scheme Other
主題 adenovirus
言語 en
主題Scheme Other
主題 retrovirus
言語 en
主題Scheme Other
主題 liver transplantation
内容記述
内容記述タイプ Other
内容記述 In this model of liver transplantation, liver grafts were transfected with the El-deleted, replication-defective adenovirus vectors encoding the LacZ gene driven by the human CMV promoter, or the replication-defective retrovirus vectors encoding the human IL-7 and neomycin phosphotransferase genes. Liver grafts were perfused ex-vivo with either of these two vectors during cold preservation time. In adenovirus-mediated gene transfer, positive staining of the hepatocyte was recognized predominantly around the portal triads at post-operative day 2. Positive staining was observed until 14 days after transfection. In a retrovirus-mediated gene transfection, gene transfection was tested both in a whole-size and a reduced-size graft implantation of the liver because the retrovirus vector requires a replication of the target cells for gene transfection. In conclusion, adenovirus-mediated gene transfection to the liver graft during cold preservation time resulted in an effective expression of the transfected sequence. On the other hand, retrovirus vector required the reduction of the liver graft for a successful gene transfection.
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346888X
収録物識別子タイプ ISSN
収録物識別子 0289-1530
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 17, 号 1, p. 21-24, 発行日 1997
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